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Cancer Gene Profiling

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Robert Grützmann

    1. Department of Surgery, Universitätsklinikum Erlangen, Erlangen, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Christian Pilarsky

    1. Department of Surgery, TU-Dresden, Dresden, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step details essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1381)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Factors Affecting the Use of Human Tissues in Biomedical Research: Implications in the Design and Operation of a Biorepository

    • Daniel S. Atherton, Katherine C. Sexton, Dennis Otali, Walter C. Bell, William E. Grizzle
    Pages 1-38

  3. Tissue Microdissection

    • Anja Rabien, Glen Kristiansen
    Pages 39-52

  4. Tissue Microarrays

    • Ana-Maria Dancau, Ronald Simon, Martina Mirlacher, Guido Sauter
    Pages 53-65

  5. Gene Expression Analysis in the Age of Mass Sequencing: An Introduction

    • Christian Pilarsky, Lahiri Kanth Nanduri, Janine Roy
    Pages 67-73

  6. Quantitative DNA Methylation Profiling in Cancer

    • Ole Ammerpohl, Andrea Haake, Julia Kolarova, Reiner Siebert
    Pages 75-92

  7. Single-Nucleotide Polymorphism to Associate Cancer Risk

    • Victoria Shaw, Katie Bullock, William Greenhalf
    Pages 93-110

  8. Exosomes in Cancer Disease

    • Margot Zöller
    Pages 111-149

  9. MicroRNA (miRNA) Profiling

    • Lu Gao, Feng Jiang
    Pages 151-161

  10. Cancer Gene Profiling for Response Prediction

    • B. Michael Ghadimi, Peter Jo
    Pages 163-179

  11. Target Gene Discovery for Novel Therapeutic Agents in Cancer Treatment

    • Sanjay Tiwari, Ole Ammerpohl, Holger Kalthoff
    Pages 181-200

  12. Application of Proteomics in Cancer Biomarker Discovery: GeLC-MS/MS

    • Pedro R. Cutillas, Tatjana Crnogorac-Jurcevic
    Pages 201-209

  13. Meta-analysis of Cancer Gene Profiling Data

    • Janine Roy, Christof Winter, Michael Schroeder
    Pages 211-222

  14. Reproducible, Scalable Fusion Gene Detection from RNA-Seq

    • Vladan Arsenijevic, Brandi N. Davis-Dusenbery
    Pages 223-237

  15. Transcriptome Sequencing for the Detection of Chimeric Transcripts

    • Hsueh-Ting Chu
    Pages 239-253

  16. Back Matter

    Pages 255-256
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Keywords

About this book

This second edition volume brings together the experiences of leading scientists in the discipline of cancer gene profiling. Because cancer genes can be profiled in many different ways, Cancer Gene Profiling: Methods and Protocols, Second Edition explores different techniques and approaches needed to understand the key stages of cancer development, as using only one technique would be insufficient. This book provides readers with an overview of the state-of-the-art methods that will enable them to perform these experiments, and demonstrates the kind of analysis that is possible in our current biomedical research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Cancer Gene Profiling: Methods and Protocols, Second Edition is a great resource for any student or practitioner with an interest in cancer gene profiling, and can be used in any well-equipped research laboratory.

Editors and Affiliations

  • Department of Surgery, Universitätsklinikum Erlangen, Erlangen, Germany

    Robert Grützmann

  • Department of Surgery, TU-Dresden, Dresden, Germany

    Christian Pilarsky

Bibliographic Information

  • Book Title: Cancer Gene Profiling

  • Book Subtitle: Methods and Protocols

  • Editors: Robert Grützmann, Christian Pilarsky

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3204-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3203-0Published: 15 December 2015

  • Softcover ISBN: 978-1-4939-7997-4Published: 27 March 2019

  • eBook ISBN: 978-1-4939-3204-7Published: 14 December 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 256

  • Number of Illustrations: 24 b/w illustrations, 24 illustrations in colour

  • Topics: Cancer Research, Gene Expression

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