Skip to main content
  • Book
  • © 2016

Lipid Signaling Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1376)

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
  2. Fluorescent Assays for Ceramide Synthase Activity

    • Timothy A. Couttas, Anthony S. Don
    Pages 23-33
  3. Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-Kinase Type II Alpha

    • Avanti Gokhale, Pearl V. Ryder, Stephanie A. Zlatic, Victor Faundez
    Pages 35-42
  4. Measurement of Long-Chain Fatty Acyl-CoA Synthetase Activity

    • Joachim Füllekrug, Margarete Poppelreuther
    Pages 43-53
  5. Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity

    • Matthew C. Gage, Benoit Pourcet, Inés Pineda-Torra
    Pages 77-85
  6. Isolation and Analysis of Detergent-Resistant Membrane Fractions

    • Massimo Aureli, Sara Grassi, Sandro Sonnino, Alessandro Prinetti
    Pages 107-131
  7. Detection of Isolated Mitochondria-Associated ER Membranes Using the Sigma-1 Receptor

    • Abasha Lewis, Shang-Yi Tsai, Tsung-Ping Su
    Pages 133-140
  8. Analyzing Protein–Phosphoinositide Interactions with Liposome Flotation Assays

    • Ricarda A. Busse, Andreea Scacioc, Amanda M. Schalk, Roswitha Krick, Michael Thumm, Karin Kühnel
    Pages 155-162
  9. Guidelines for the Use of Protein Domains in Acidic Phospholipid Imaging

    • Matthieu Pierre Platre, Yvon Jaillais
    Pages 175-194

About this book

In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. Lipid Signaling Protocols, Second Edition guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the technical considerations, challenges and potential pitfalls associated with using these laboratory-based approaches. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Lipid Signaling Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

 

Editors and Affiliations

  • University College of London School of Life and Medical Sciences, London, United Kingdom

    Mark G. Waugh

Bibliographic Information

  • Book Title: Lipid Signaling Protocols

  • Editors: Mark G. Waugh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3170-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3169-9Published: 10 November 2015

  • Softcover ISBN: 978-1-4939-5007-2Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3170-5Published: 09 November 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 257

  • Topics: Cell Biology, Membrane Biology, Lipidology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access