Overview
- Includes cutting-edge methods and protocols for the study of Staphylococci genetic manipulation
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1373)
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Table of contents (20 protocols)
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Front Matter
Keywords
About this book
This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using these methods, the authors have been successful at making mutants that span individual single-nucleotide changes in the chromosome to whole genome mutant libraries. The latter strategy provides a useful tool for high-throughput screening, while single nucleotide changes are an elegant way of teasing apart the importance of single nucleotides in gene expression or to create specific amino acid substitutions to examine protein function. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, The Genetic Manipulation of Staphylococci: Methods and Protocols serves as a vital resource and guide to scientists in the Staphylococcus community as they pursue their studies on these bacteria.
Editors and Affiliations
Bibliographic Information
Book Title: The Genetic Manipulation of Staphylococci
Book Subtitle: Methods and Protocols
Editors: Jeffrey L. Bose
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3158-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3157-6Published: 20 October 2015
Softcover ISBN: 978-1-4939-4900-7Published: 23 August 2016
eBook ISBN: 978-1-4939-3158-3Published: 20 October 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 186
Number of Illustrations: 16 b/w illustrations, 7 illustrations in colour
Topics: Microbiology, Gene Expression