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Plant Cell Division

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Marie-Cécile Caillaud

    1. Institut National de la Recherche Agronomique, Ecole Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, Laboratoire de Reproduction et Développement des Plantes, Centre National de la Recherche Scientifique, Lyon, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1370)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Cell Cultures for Cell Division Studies

    1. Front Matter

      Pages 1-1
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    2. Synchronization of Green Algae by Light and Dark Regimes for Cell Cycle and Cell Division Studies

      • Monika Hlavová, Milada Vítová, Kateřina Bišová
      Pages 3-16

    3. Plant Cell Division Analyzed by Transient Agrobacterium-Mediated Transformation of Tobacco BY-2 Cells

      • Henrik Buschmann
      Pages 17-25

  3. Cell Cycle and Mitosis

    1. Front Matter

      Pages 27-27
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    2. Identifying Gene Regulatory Networks in Arabidopsis by In Silico Prediction, Yeast-1-Hybrid, and Inducible Gene Profiling Assays

      • Erin E. Sparks, Philip N. Benfey
      Pages 29-50

    3. Cytrap Marker Systems for In Vivo Visualization of Cell Cycle Progression in Arabidopsis

      • Shiori Sugamata Aki, Masaaki Umeda
      Pages 51-57

    4. Imaging Nuclear Morphology and Organization in Cleared Plant Tissues Treated with Cell Cycle Inhibitors

      • José Dijair Antonino de Souza Junior, Maria Fatima Grossi de Sa, Gilbert Engler, Janice de Almeida Engler
      Pages 59-68

    5. Isolation of Plant Nuclei at Defined Cell Cycle Stages Using EdU Labeling and Flow Cytometry

      • Emily E. Wear, Lorenzo Concia, Ashley M. Brooks, Emily A. Markham, Tae-Jin Lee, George C. Allen et al.
      Pages 69-86

    6. Preparation and Fluorescent Analysis of Plant Metaphase Chromosomes

      • Trude Schwarzacher
      Pages 87-103

    7. Fluorescence In Situ Hybridization on Rice Chromosomes

      • Yafei Li, Zhukuan Cheng
      Pages 105-112

  4. Imaging Plant Cell Division

    1. Front Matter

      Pages 113-113
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    2. Dynamics of the Plant Nuclear Envelope During Cell Division

      • David E. Evans, Katja Graumann
      Pages 115-126

    3. Immunolabeling of Nuclei/Chromosomes in Arabidopsis thaliana

      • Michael Sandmann, Joerg Fuchs, Inna Lermontova
      Pages 127-135

    4. In Vivo Imaging of Microtubule Organization in Dividing Giant Cell

      • Marie-Cécile Caillaud, Bruno Favery
      Pages 137-144

  5. Cell Division and Morphogenesis

    1. Front Matter

      Pages 145-145
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    2. Real-Time Lineage Analysis to Study Cell Division Orientation in the Arabidopsis Shoot Meristem

      • Cory J. Tobin, Elliot M. Meyerowitz
      Pages 147-167

    3. Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis

      • Anita Kazda, Svetlana Akimcheva, J. Matthew Watson, Karel Riha
      Pages 169-182

    4. Studying Cell Division Plane Positioning in Early-Stage Embryos

      • Katia Belcram, Jean-Christophe Palauqui, Martine Pastuglia
      Pages 183-195

  6. Cytokinesis

    1. Front Matter

      Pages 197-197
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Keywords

About this book

This volume aims to present a large panel of techniques for the study of Plant Cell Division. Plant Cell Division: Methods and Protocols captures basic experimental protocols that are commonly used to study plant cell division processes, as well as more innovative procedures. Chapters are split into five parts covering several different aspect of plant cell division such as,   cell cultures for cell division studies, cell cycle progression and mitosis, imaging plant cell division, cell division and morphogenesis, and cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Cell Division: Methods and Protocols is a valuable tool for the study of plant cell division at both the cellular and molecular levels, and in the context of plant development.

Editors and Affiliations

  • Institut National de la Recherche Agronomique, Ecole Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, Laboratoire de Reproduction et Développement des Plantes, Centre National de la Recherche Scientifique, Lyon, France

    Marie-Cécile Caillaud

Bibliographic Information

  • Book Title: Plant Cell Division

  • Book Subtitle: Methods and Protocols

  • Editors: Marie-Cécile Caillaud

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3142-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3141-5Published: 11 December 2015

  • Softcover ISBN: 978-1-4939-7988-2Published: 30 March 2019

  • eBook ISBN: 978-1-4939-3142-2Published: 11 December 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 243

  • Number of Illustrations: 13 b/w illustrations, 35 illustrations in colour

  • Topics: Plant Sciences, Cell Culture

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