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Estrogen Receptors

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Kathleen M. Eyster

    1. University of South Dakota Sanford School of Medicine, Vermillion, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1366)

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Table of contents (47 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. The Estrogen Receptors: An Overview from Different Perspectives

    • Kathleen M. Eyster
    Pages 1-10

  3. Competitive Binding Assay for the G-Protein-Coupled Receptor 30 (GPR30) or G-Protein-Coupled Estrogen Receptor (GPER)

    • Thomas Thekkumkara, Russell Snyder, Vardan T. Karamyan
    Pages 11-17

  4. The Use of Real-Time Reverse Transcription-PCR for Assessing Estrogen Receptor and Estrogen-Responsive Gene Expression

    • Michelle L. Booze, Kathleen M. Eyster
    Pages 19-28

  5. Bioinformatics Analysis of Estrogen-Responsive Genes

    • Adam E. Handel
    Pages 29-39Open Access

  6. Electrophoretic Mobility Shift Assay (EMSA) and Supershift Assay of Cytochrome P450 2B6 in Response to Estrogen

    • Kwi Hye Koh, Hyunyoung Jeong
    Pages 41-51

  7. Chromatin Immunoprecipitation Assay to Identify Genomic Binding Sites of Regulatory Factors

    • Meike Wagner, Johannes Jung, Michael Koslowski, Özlem Türeci, Vijay K. Tiwari, Ugur Sahin
    Pages 53-65

  8. Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of Greb1 in TM4 Sertoli Cells

    • Jing Lin, Zhenmin Lei
    Pages 67-77

  9. Chromatin Immunoprecipitation-Sequencing (ChIP-seq) for Mapping of Estrogen Receptor-Chromatin Interactions in Breast Cancer

    • Kelly A. Holmes, Gordon D. Brown, Jason S. Carroll
    Pages 79-98

  10. RNA-Seq Experiment and Data Analysis

    • Hanquan Liang, Erliang Zeng
    Pages 99-114

  11. DNA Microarray Analysis of Estrogen-Responsive Genes

    • Kathleen M. Eyster
    Pages 115-129

  12. Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two-Dimensional Liquid Chromatography and Tandem Mass Spectrometry

    • Eduardo A. Callegari
    Pages 131-148

  13. Assessment of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate Endometrium, Placenta, and Fetal Adrenal in Response to Estrogen

    • Thomas W. Bonagura, Jeffery S. Babischkin, Gerald J. Pepe, Eugene D. Albrecht
    Pages 149-161

  14. Colocalization of Estrogen Receptors with the Fluorescent Tamoxifen Derivative, FLTX1, Analyzed by Confocal Microscopy

    • Araceli Morales, Raquel Marín, Jorge Marrero-Alonso, Alicia Boto, Mario Díaz
    Pages 163-173

  15. Live-Cell Imaging of the Estrogen Receptor by Total Internal Reflection Fluorescence Microscopy

    • Kassandra Kisler, Reymundo Dominguez
    Pages 175-187

  16. In Situ Hybridization of Estrogen Receptors α and β and GPER in the Human Testis

    • Daniela Fietz, M. Bergmann, K. Hartmann
    Pages 189-205

  17. Purification of Histone Lysine Methyltransferase SMYD2 and Co-Crystallization with a Target Peptide from Estrogen Receptor α

    • Yuanyuan Jiang, Joshua Holcomb, Nicholas Spellmon, Zhe Yang
    Pages 207-217

  18. Gold Nanoparticle-Based Förster Resonance Energy Transfer (FRET) Analysis of Estrogen Receptor: DNA Interaction

    • Xiaodi Su, Khin Moh Moh Aung, Steven Lukman, Bin Liu
    Pages 219-232

  19. Analysis of Interaction of Estradiol with Estrogen Receptor by NMR Spectroscopy

    • M. K. Thakur, V. Paramanik
    Pages 233-239

  20. Application of Circular Dichroism Spectroscopy to the Analysis of the Interaction Between the Estrogen Receptor Alpha and Coactivators: The Case of Calmodulin

    • Emeric Miclet, Sandrine Bourgoin-Voillard, Cillian Byrne, Yves Jacquot
    Pages 241-259
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Keywords

About this book

This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen.

The chapter 'Bioinformatics Analysisof Estrogen-Responsive Genes' is open access under a CC BY 4.0 license. 

Editors and Affiliations

  • University of South Dakota Sanford School of Medicine, Vermillion, USA

    Kathleen M. Eyster

About the editor

Kathleen (Kathy) Eyster Professor of Physiology and Pharmacology Sanford School of Medicine of the University of South Dakota 605-677-5159

Bibliographic Information

  • Book Title: Estrogen Receptors

  • Book Subtitle: Methods and Protocols

  • Editors: Kathleen M. Eyster

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3127-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3126-2Published: 20 November 2015

  • Softcover ISBN: 978-1-4939-5006-5Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3127-9Published: 19 November 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 576

  • Number of Illustrations: 41 b/w illustrations, 87 illustrations in colour

  • Topics: Biochemistry, general, Endocrinology

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