Skip to main content
  • Book
  • © 2016

Cytoskeleton Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1365)

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiii
  2. Imaging the Cytoskeleton in Live and Fixed Cells

    1. Front Matter

      Pages 1-1
    2. Live-Cell Imaging of Mitochondria and the Actin Cytoskeleton in Budding Yeast

      • Ryo Higuchi-Sanabria, Theresa C. Swayne, Istvan R. Boldogh, Liza A. Pon
      Pages 25-62
    3. Imaging of the Actin Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells

      • Ryo Higuchi-Sanabria, Theresa C. Swayne, Istvan R. Boldogh, Liza A. Pon
      Pages 63-81
    4. Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells

      • Derek A. Applewhite, Christine A. Davis, Eric R. Griffis, Omar A. Quintero
      Pages 83-97
    5. Fluorescence Imaging of the Cytoskeleton in Plant Roots

      • Julia Dyachok, Ana Paez-Garcia, Cheol-Min Yoo, Karuppaiah Palanichelvam, Elison B. Blancaflor
      Pages 139-153
    6. Microtubules in Plant Cells: Strategies and Methods for Immunofluorescence, Transmission Electron Microscopy, and Live Cell Imaging

      • Katherine Celler, Miki Fujita, Eiko Kawamura, Chris Ambrose, Klaus Herburger, Andreas Holzinger et al.
      Pages 155-184
  3. Cell and Organelle Motility

    1. Front Matter

      Pages 263-263
    2. Quantitative Motion Analysis in Two and Three Dimensions

      • Deborah J. Wessels, Daniel F. Lusche, Spencer Kuhl, Amanda Scherer, Edward Voss, David R. Soll
      Pages 265-292
    3. Measurement of Cell Motility Using Microgrooved Substrates

      • James H.-C. Wang, Guangyi Zhao, Bin Li
      Pages 293-299
    4. The Study of Cell Motility by Cell Traction Force Microscopy (CTFM)

      • James H.-C. Wang, Guangyi Zhao, Bin Li
      Pages 301-313

About this book

The third edition of this volume focuses on experimental models that are useful for investigating various aspects of cytoskeleton structure and function. Animal, plant, protist, and fungal models highlight twenty-four chapters that provide detailed protocols for live and fixed-cell imaging, dynamics of cytoskeleton components, cell and organelle motility, and genetics and proteomics. Written in the highly successful Methods in Molecular Biology series format, protocols in each chapter are up-to-date menus organized in a useful step-by-step format appropriate for novice and established investigators. Each chapter is equipped with a valuable notes section that provides a troubleshooting guide and helpful, and often unpublished, technical information aimed at ensuring success with implementation of the protocols.

Authoritative and thorough, Cytoskeleton Methods and Protocols, Third Edition helps researchers expand their understanding of cytoskeleton structure and function.

Editors and Affiliations

  • Brooklyn College, Brooklyn, USA

    Ray H. Gavin

Bibliographic Information

  • Book Title: Cytoskeleton Methods and Protocols

  • Editors: Ray H. Gavin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3124-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3123-1Published: 24 October 2015

  • Softcover ISBN: 978-1-4939-4975-5Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3124-8Published: 23 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIII, 431

  • Number of Illustrations: 53 b/w illustrations, 38 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access