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  • © 2016

Plant Signal Transduction

Methods and Protocols

  • Includes updated and cutting-edge methods and protocols for studying plant signal transduction
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1363)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xv
  2. Suppressor Screens in Arabidopsis

    • Xin Li, Yuelin Zhang
    Pages 1-8
  3. Culture of the Tomato Micro-Tom Cultivar in Greenhouse

    • Christophe Rothan, Daniel Just, Lucie Fernandez, Isabelle Atienza, Patricia Ballias, Martine Lemaire-Chamley
    Pages 57-64
  4. Preparation of Epidermal Peels and Guard Cell Protoplasts for Cellular, Electrophysiological, and -Omics Assays of Guard Cell Function

    • Mengmeng Zhu, Byeong Wook Jeon, Sisi Geng, Yunqing Yu, Kelly Balmant, Sixue Chen et al.
    Pages 89-121
  5. Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions

    • Natsumi Maruta, Yuri Trusov, Jose R. Botella
    Pages 145-154
  6. Analysis of Protein–Lipid Interactions Using Purified C2 Domains

    • Jessica Pérez-Sancho, Arnaldo L. Schapire, Miguel A. Botella, Abel Rosado
    Pages 175-187
  7. Assessing Kinase Activity in Plants with In-Gel Kinase Assays

    • Pengcheng Wang, Jian-Kang Zhu
    Pages 189-197
  8. Competitive Index: Mixed Infection-Based Virulence Assays for Genetic Analysis in Pseudomonas syringae-Plant Interactions

    • Alberto P. Macho, José S. Rufián, Javier Ruiz-Albert, Carmen R. Beuzón
    Pages 209-217
  9. Simplified Assays for Evaluation of Resistance to Alternaria brassicicola and Turnip Mosaic Virus

    • Yuri Trusov, Ralf G. Dietzgen, Natsumi Maruta, Jose R. Botella
    Pages 219-228
  10. Simultaneous Determination of Plant Hormones by GC-TOF-MS

    • José G. Vallarino, Sonia Osorio
    Pages 229-237

About this book

This fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of ‘classic’ as well as newly developed approaches. These detailed approaches expand into the fields of molecular biology, biochemistry, physiology, cell biology, genetics, and genomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and up-to-date, Plant Signal Transduction: Methods and Protocols, Second Edition serves as an ideal guide for researchers exploring the vast array of signals produced by plants to ensure their survival.

Editors and Affiliations

  • The University of Queensland, St. Lucia, Australia

    Jose R. Botella

  • Universidad de Málaga, Málaga, Spain

    Miguel A. Botella

Bibliographic Information

  • Book Title: Plant Signal Transduction

  • Book Subtitle: Methods and Protocols

  • Editors: Jose R. Botella, Miguel A. Botella

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3115-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3114-9Published: 18 November 2015

  • Softcover ISBN: 978-1-4939-4959-5Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3115-6Published: 17 November 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 241

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 79.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 99.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access