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  • © 2016

Yeast Functional Genomics

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1361)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
  2. Using RNA-seq for Analysis of Differential Gene Expression in Fungal Species

    • Can Wang, Markus S. Schröder, Stephen Hammel, Geraldine Butler
    Pages 1-40
  3. Enhancing Structural Annotation of Yeast Genomes with RNA-Seq Data

    • Hugo Devillers, Nicolas Morin, Cécile Neuvéglise
    Pages 41-56
  4. Pathogen Gene Expression Profiling During Infection Using a Nanostring nCounter Platform

    • Wenjie Xu, Norma V. Solis, Scott G. Filler, Aaron P. Mitchell
    Pages 57-65
  5. Comparative Transcriptomics in Yeasts

    • Dawn A. Thompson
    Pages 67-76
  6. Translation Analysis at the Genome Scale by Ribosome Profiling

    • Agnès Baudin-Baillieu, Isabelle Hatin, Rachel Legendre, Olivier Namy
    Pages 105-124
  7. Biotin-Genomic Run-On (Bio-GRO): A High-Resolution Method for the Analysis of Nascent Transcription in Yeast

    • Antonio Jordán-Pla, Ana Miguel, Eva Serna, Vicent Pelechano, José E. Pérez-Ortín
    Pages 125-139
  8. Genome-Wide Probing of RNA Structures In Vitro Using Nucleases and Deep Sequencing

    • Yue Wan, Kun Qu, Zhengqing Ouyang, Howard Y. Chang
    Pages 141-160
  9. Genome-Wide Chromatin Immunoprecipitation in Candida albicans and Other Yeasts

    • Matthew B. Lohse, Pisiwat Kongsomboonvech, Maria Madrigal, Aaron D. Hernday, Clarissa J. Nobile
    Pages 161-184
  10. Generation and Analysis of Chromosomal Contact Maps of Yeast Species

    • Axel Cournac, Martial Marbouty, Julien Mozziconacci, Romain Koszul
    Pages 227-245
  11. Label-Free Quantitative Proteomics in Yeast

    • Thibaut Léger, Camille Garcia, Mathieu Videlier, Jean-Michel Camadro
    Pages 289-307
  12. Profiling of Yeast Lipids by Shotgun Lipidomics

    • Christian Klose, Kirill Tarasov
    Pages 309-324

About this book

This volume provides a collection of protocols for the study of DNA-DNA contact maps, replication profiles, transcription rates, RNA secondary structures, protein-RNA interactions, ribosome profiling and quantitative proteomes and metabolomes. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Yeast Functional Genomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Laboratoire de biologie computationnelle et quantitative, Sorbonne Universités, Paris, France

    Frédéric Devaux

Bibliographic Information

  • Book Title: Yeast Functional Genomics

  • Book Subtitle: Methods and Protocols

  • Editors: Frédéric Devaux

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3079-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3078-4Published: 20 October 2015

  • Softcover ISBN: 978-1-4939-5002-7Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3079-1Published: 19 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 406

  • Number of Illustrations: 26 b/w illustrations, 51 illustrations in colour

  • Topics: Microbiology, Gene Expression

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access