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Kinase Screening and Profiling

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Hicham Zegzouti

    1. Promega Corporation, Madison, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Said A. Goueli

    1. Promega Corporation, Madison, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1360)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. HTRF Kinase Assay Development and Methods in Inhibitor Characterization

    • Yong Jia, Mari Manuia, Jose Juarez
    Pages 1-18

  3. Application of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for Cancer Therapy: Expression, Purification, and High-Throughput Inhibitor Screening

    • Clint D. J. Tavares, Ashwini K. Devkota, Kevin N. Dalby, Eun Jeong Cho
    Pages 19-33

  4. Recombinant Kinase Production and Fragment Screening by NMR Spectroscopy

    • Byeonggu Han, Hee-Chul Ahn
    Pages 35-46

  5. Bioluminescence Methods for Assaying Kinases in Quantitative High-Throughput Screening (qHTS) Format Applied to Yes1 Tyrosine Kinase, Glucokinase, and PI5P4Kα Lipid Kinase

    • Mindy I. Davis, Douglas S. Auld, James Inglese
    Pages 47-58

  6. Using Bioluminescent Kinase Profiling Strips to Identify Kinase Inhibitor Selectivity and Promiscuity

    • Hicham Zegzouti, Jacquelyn Hennek, Said A. Goueli
    Pages 59-73

  7. Measuring Activity of Phosphoinositide Lipid Kinases Using a Bioluminescent ADP-Detecting Assay

    • Andrew W. Tai, Jolanta Vidugiriene
    Pages 75-85

  8. A High-Throughput Radiometric Kinase Assay

    • Krisna C. Duong-Ly, Jeffrey R. Peterson
    Pages 87-95

  9. A High-Content Assay to Screen for Modulators of EGFR Function

    • Christophe Antczak, Hakim Djaballah
    Pages 97-106

  10. Monitoring Protein Kinase Expression and Phosphorylation in Cell Lysates with Antibody Microarrays

    • Hong Zhang, Xiaoqing Shi, Steven Pelech
    Pages 107-122

  11. From Enzyme to Whole Blood: Sequential Screening Procedure for Identification and Evaluation of p38 MAPK Inhibitors

    • Silke M. Bauer, Jakub M. Kubiak, Ulrich Rothbauer, Stefan Laufer
    Pages 123-148

  12. Genetically Encoded Fluorescent Indicators to Visualize Protein Phosphorylation in Living Cells

    • Moritoshi Sato, Yoshio Umezawa
    Pages 149-156

  13. Characterization of an Engineered Src Kinase to Study Src Signaling and Biology

    • Leanna R. Gentry, Andrei V. Karginov, Klaus M. Hahn, Channing J. Der
    Pages 157-167

  14. Screening One-Bead-One-Compound Peptide Libraries for Optimal Kinase Substrates

    • Thi B. Trinh, Dehua Pei
    Pages 169-181

  15. Determination of the Substrate Specificity of Protein Kinases with Peptide Micro- and Macroarrays

    • Shenshen Lai, Dirk F. H. Winkler, Hong Zhang, Steven Pelech
    Pages 183-202

  16. Rapid Identification of Protein Kinase Phosphorylation Site Motifs Using Combinatorial Peptide Libraries

    • Chad J. Miller, Benjamin E. Turk
    Pages 203-216

  17. Back Matter

    Pages 217-223
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Keywords

About this book

This volume focuses on screening and profiling kinase inhibitors or activators. Chapters in this book cover several facets of the drug discovery processes starting with target identification, and assay development and screening chemical libraries for hit identification and lead optimization. The profiling portion of Kinase Screening and Profiling: Methods and Protocols discusses several strategies and techniques that are required to minimize off target hits and reduce cellular toxicities that are caused by liability kinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Timely and authoritative, Kinase Screening and Profiling: Methods and Protocols, will benefit scientists and researchers who are interested in exploring the role of kinases in academic, as well as pharmaceutical research.

Editors and Affiliations

  • Promega Corporation, Madison, USA

    Hicham Zegzouti, Said A. Goueli

Bibliographic Information

  • Book Title: Kinase Screening and Profiling

  • Book Subtitle: Methods and Protocols

  • Editors: Hicham Zegzouti, Said A. Goueli

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3073-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3072-2Published: 27 October 2015

  • Softcover ISBN: 978-1-4939-4923-6Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3073-9Published: 26 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 223

  • Number of Illustrations: 19 b/w illustrations, 26 illustrations in colour

  • Topics: Protein Science

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