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Candida Species

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Richard Calderone

    1. Dept Microbiology & Immunology, Georgetown University Medical Center, Washington, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Ronald Cihlar

    1. Dept of Microbiology & Immunology, Georgetown University Medical Center, Washington, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1356)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
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  2. Molecular Analyses of Pathogenesis

    1. Front Matter

      Pages 1-1
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    2. How to Use the Candida Genome Database

      • Marek S. Skrzypek, Jonathan Binkley, Gavin Sherlock
      Pages 3-15

    3. Transcriptional Profiling of Candida albicans in the Host

      • Kearney T. W. Gunsalus, Carol A. Kumamoto
      Pages 17-29

    4. SRYTH: A New Yeast Two-Hybrid Method

      • Jaideep Mallick, Gregor Jansen, Cunle Wu, Malcolm Whiteway
      Pages 31-41

    5. Chromatin Immunoprecipitation (ChIP) Assay in Candida albicans

      • Sreyoshi Mitra, Laxmi Shanker Rai, Gautam Chatterjee, Kaustuv Sanyal
      Pages 43-57

    6. Assessing Mitochondrial Functions in Candida albicans

      • Dongmei Li, Richard Calderone
      Pages 59-67

    7. Imaging Candida Infections in the Host

      • Dhammika H. Navarathna, David D. Roberts, Jeeva Munasinghe, Martin J. Lizak
      Pages 69-78

    8. Identification of Secreted Candida Proteins Using Mass Spectrometry

      • Emilia Gómez-Molero, Henk L. Dekker, Albert D. de Boer, Piet W. J. de Groot
      Pages 79-94

    9. In Vitro Models for Candida Biofilm Development

      • Bastiaan P. Krom, Hubertine M. E. Willems
      Pages 95-105

    10. The Fungal Biome of the Oral Cavity

      • Jyotsna Chandra, Mauricio Retuerto, Pranab K. Mukherjee, Mahmoud Ghannoum
      Pages 107-135

    11. Experimental Models of C. albicans-Streptococcal Co-infection

      • Takanori Sobue, Patricia Diaz, Hongbin Xu, Martinna Bertolini, Anna Dongari-Bagtzoglou
      Pages 137-152

    12. Staphylococcus–Candida Interaction Models: Antibiotic Resistance Testing and Host Interactions

      • Nina Scheres, Bastiaan P. Krom
      Pages 153-161

  3. Drug Discovery and Susceptibility Testing

    1. Front Matter

      Pages 163-163
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    2. Genetic Screens for Determination of Mechanism of Action

      • Françoise Gay-Andrieu, Deepu Alex, Richard Calderone
      Pages 165-172

    3. Microbroth Dilution Susceptibility Testing of Candida species

      • Randal J. Kuykendall, Shawn R. Lockhart
      Pages 173-181

    4. Biofilms and Antifungal Susceptibility Testing

      • Maria Simitsopoulou, Athanasios Chatzimoschou, Emmanuel Roilides
      Pages 183-197

    5. Typing Candida Species Using Microsatellite Length Polymorphism and Multilocus Sequence Typing

      • Dea Garcia-Hermoso, Marie Desnos-Ollivier, Stéphane Bretagne
      Pages 199-214

    6. Diagnostic Methods for Detection of Blood-Borne Candidiasis

      • Cornelius J. Clancy, M. Hong Nguyen
      Pages 215-238

  4. Back Matter

    Pages 239-240
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Keywords

About this book

This volume provides detailed discussion of a variety of important techniques that researchers use to study fungal molecular biology and pathogenesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Candida Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Dept Microbiology & Immunology, Georgetown University Medical Center, Washington, USA

    Richard Calderone

  • Dept of Microbiology & Immunology, Georgetown University Medical Center, Washington, USA

    Ronald Cihlar

Bibliographic Information

  • Book Title: Candida Species

  • Book Subtitle: Methods and Protocols

  • Editors: Richard Calderone, Ronald Cihlar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3052-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3051-7Published: 10 November 2015

  • Softcover ISBN: 978-1-4939-7980-6Published: 27 March 2019

  • eBook ISBN: 978-1-4939-3052-4Published: 30 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 240

  • Number of Illustrations: 6 b/w illustrations, 49 illustrations in colour

  • Topics: Bacteriology

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