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  • © 2016

HIV Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1354)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
  2. HIV-1 Replication—Early Events

    1. Front Matter

      Pages 1-1
    2. HIV-1 Capsid Stabilization Assay

      • Thomas Fricke, Felipe Diaz-Griffero
      Pages 39-47
  3. HIV-1 RNA Structure and RNA-Protein Interactions

    1. Front Matter

      Pages 89-89
    2. Novel Biochemical Tools for Probing HIV RNA Structure

      • Jason W. Rausch, Joanna Sztuba-Solinska, Sabrina Lusvarghi, Stuart F. J. Le Grice
      Pages 91-117
    3. Analysis of HIV-1 Gag-RNA Interactions in Cells and Virions by CLIP-seq

      • Sebla B. Kutluay, Paul D. Bieniasz
      Pages 119-131
  4. HIV-1 Replication—Post-integration Events

    1. Front Matter

      Pages 147-147
    2. Methods for the Analyses of Inhibitor-Induced Aberrant Multimerization of HIV-1 Integrase

      • Jacques J. Kessl, Amit Sharma, Mamuka Kvaratskhelia
      Pages 149-164
    3. Quantification of HIV-1 Gag Localization Within Virus Producer Cells

      • Annalena La Porte, Ganjam V. Kalpana
      Pages 165-174
  5. Studying HIV-1 Replication and Pathogenesis in Animal Models

    1. Front Matter

      Pages 187-187
    2. Visualizing the Behavior of HIV-Infected T Cells In Vivo Using Multiphoton Intravital Microscopy

      • Radwa Sharaf, Thorsten R. Mempel, Thomas T. Murooka
      Pages 189-201
    3. Modeling HIV-1 Mucosal Transmission and Prevention in Humanized Mice

      • Milena Veselinovic, Paige Charlins, Ramesh Akkina
      Pages 203-220
    4. High-Throughput Humanized Mouse Models for Evaluation of HIV-1 Therapeutics and Pathogenesis

      • Tynisha Thomas, Kieran Seay, Jian Hua Zheng, Cong Zhang, Christina Ochsenbauer, John C. Kappes et al.
      Pages 221-235

About this book

This third edition volume expands upon the previous editions with new research topics and numerous leading-edge HIV protocols. This book contains chapters that cover various aspects of HIV-1 replication and are helpful for investigating the mechanisms of HIV-1 replication or pathogenesis. The chapters are grouped into six sections: HIV early events; HIV-1 RNA structure and RNA-protein interactions; HIV-1 post-integration events; HIV-1 pathogenesis in animal models; tools to study HIV-1 latency; and Neuro AIDS. Chapters are written by research experts who have diligently developed techniques, improved them, simplified them, or applied them to their own HIV research. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, HIV Protocols, Third Edition is a great resource for HIV research, and contains many significant tips and tricks rarely mentioned in standard protocols.

Editors and Affiliations

  • Department of Microbiology & Immunology, Albert Einstein College of Medicine, BRONX, USA

    Vinayaka R. Prasad

  • Department of Genetics, Albert Einstein College of Medicine, BRONX, USA

    Ganjam V. Kalpana

Bibliographic Information

  • Book Title: HIV Protocols

  • Editors: Vinayaka R. Prasad, Ganjam V. Kalpana

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3046-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3045-6Published: 29 December 2015

  • Softcover ISBN: 978-1-4939-7979-0Published: 27 March 2019

  • eBook ISBN: 978-1-4939-3046-3Published: 29 December 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIV, 383

  • Number of Illustrations: 27 b/w illustrations, 36 illustrations in colour

  • Topics: Immunology, Virology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access