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Peptide Microarrays

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Marina Cretich

    1. Consiglio Nazionale delle Ricerche, Milano, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Marcella Chiari

    1. Consiglio Nazionale delle Ricerche, Milano, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for peptide microarray study
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1352)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Peptide Arrays: Cutting-Edge Methods and Technology

    1. Front Matter

      Pages 1-1
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    2. Peptide Arrays on Planar Supports

      • Victor Eduardo Tapia Mancilla, Rudolf Volkmer
      Pages 3-17

    3. High-Throughput Microarray Incubations Using Multi-Well Chambers

      • Johannes Zerweck, Ulf Reimer, Janina Jansong, Nikolaus Pawlowski, Christoph Tersch, Maren Eckey et al.
      Pages 19-26

    4. Analysis of Protein Tyrosine Kinase Specificity Using Positional Scanning Peptide Microarrays

      • Yang Deng, Benjamin E. Turk
      Pages 27-34

    5. Secondary Structure Determination of Peptides and Proteins After Immobilization

      • Stella H. North, Chris R. Taitt
      Pages 35-50

    6. Peptides and Anti-peptide Antibodies for Small and Medium Scale Peptide and Anti-peptide Affinity Microarrays: Antigenic Peptide Selection, Immobilization, and Processing

      • Fan Zhang, Andrea Briones, Mikhail Soloviev
      Pages 51-66

    7. Low-Cost Peptide Microarrays for Mapping Continuous Antibody Epitopes

      • Ryan McBride, Steven R. Head, Phillip Ordoukhanian, Mansun Law
      Pages 67-83

    8. The Peptide Microarray-Based Resonance Light Scattering Assay for Sensitively Detecting Intracellular Kinase Activity

      • Tao Li, Xia Liu, Dianjun Liu, Zhenxin Wang
      Pages 85-96

    9. Anomalous Reflection of Gold: A Novel Platform for Biochips

      • Amir Syahir, Kin-ya Tomizaki, Kotaro Kajikawa, Hisakazu Mihara
      Pages 97-110

    10. High-Throughput Peptide Screening on a Bimodal Imprinting Chip Through MS-SPRi Integration

      • Weizhi Wang, Qiaojun Fang, Zhiyuan Hu
      Pages 111-125

    11. Analyzing Peptide Microarray Data with the R pepStat Package

      • Gregory Imholte, Renan Sauteraud, Raphael Gottardo
      Pages 127-142

  3. Chemoselective Strategies to Peptide Immobilization

    1. Front Matter

      Pages 143-143
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    2. Chemoselective Strategies to Peptide and Protein Bioprobes Immobilization on Microarray Surfaces

      • Alessandro Gori, Renato Longhi
      Pages 145-156

    3. Manufacturing of Peptide Microarrays Based on Catalyst-Free Click Chemistry

      • Denis Prim, Marc E. Pfeifer
      Pages 157-166

    4. Clickable Polymeric Coating for Oriented Peptide Immobilization

      • Laura Sola, Alessandro Gori, Marina Cretich, Chiara Finetti, Caterina Zilio, Marcella Chiari
      Pages 167-182

    5. Oriented Peptide Immobilization on Microspheres

      • Lisa C. Shriver-Lake, George P. Anderson, Chris R. Taitt
      Pages 183-197

    6. A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis

      • Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara
      Pages 199-210

  4. Peptide Microarrays for Medical Applications

    1. Front Matter

      Pages 211-211
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    2. Peptide Microarrays for Medical Applications in Autoimmunity, Infection, and Cancer

      • Carsten Grötzinger
      Pages 213-221
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Keywords

About this book

This expert volume provides insights into the technological fundamentals together with a comprehensive overview of the potentialities of peptide microarray technology in basic research and clinical assays. Advancements made in recent years in peptide library synthesis, immobilization chemistry and array production have created a foundation from which different new applications are derived, extending the ways in which peptide microarray technology is applied every day. Divided into three sections, the book covers cutting-edge methods and technology, chemoselective strategies for peptide immobilization, and peptide microarrays for medical applications. Written for the high successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.

Fully updated and authoritative, Peptide Microarrays: Methods and Protocols, Second Edition seeks to encourage scientists to apply current peptide array protocols to the study of interesting new biochemical and medical questions and to assist researchers aiming at developing new methods to further develop peptide microarray technology.

Editors and Affiliations

  • Consiglio Nazionale delle Ricerche, Milano, Italy

    Marina Cretich, Marcella Chiari

Bibliographic Information

  • Book Title: Peptide Microarrays

  • Book Subtitle: Methods and Protocols

  • Editors: Marina Cretich, Marcella Chiari

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3037-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3036-4Published: 22 October 2015

  • Softcover ISBN: 978-1-4939-4968-7Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3037-1Published: 21 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 298

  • Number of Illustrations: 25 b/w illustrations, 52 illustrations in colour

  • Topics: Immunology, Proteomics

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