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Vaccine Technologies for Veterinary Viral Diseases

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Alejandro Brun

    1. CISA-INIA, Valdeolmos, Spain

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  • Includes cutting-edge methods and protocols for the study of vaccine technologies applied to veterinary viral diseases
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1349)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Vaccines and Vaccination for Veterinary Viral Diseases: A General Overview

    • Alejandro Brun
    Pages 1-24

  3. Using IC-Tagging Methodology for Production and Purification of Epitope-Loaded Protein Microspheres for Vaccination

    • Natalia Barreiro-Piñeiro, Rebeca Menaya-Vargas, Alberto Brandariz-Núñez, Iria Otero-Romero, Irene Lostalé-Seijo, Javier Benavente et al.
    Pages 25-34

  4. Plant-Based Vaccine Antigen Production

    • Hoang Trong Phan, Udo Conrad
    Pages 35-47

  5. DNA Vaccines: Experiences in the Swine Model

    • Francesc Accensi, Fernando Rodríguez, Paula L. Monteagudo
    Pages 49-62

  6. Novel Adjuvants and Immunomodulators for Veterinary Vaccines

    • Peter M. H. Heegaard, Yongxiang Fang, Gregers Jungersen
    Pages 63-82

  7. Polymerase Mechanism-Based Method of Viral Attenuation

    • Cheri A. Lee, Avery August, Jamie J. Arnold, Craig E. Cameron
    Pages 83-104

  8. BacMam Platform for Vaccine Antigen Delivery

    • Günther M. Keil, Reiko Pollin, Claudia Müller, Katrin Giesow, Horst Schirrmeier
    Pages 105-119

  9. Laboratory-Scale Production of Replication-Deficient Adenovirus Vectored Vaccines

    • Susan J. Morris, Alison V. Turner, Nicola Green, George M. Warimwe
    Pages 121-135

  10. Generation of Recombinant Modified Vaccinia Virus Ankara Encoding VP2, NS1, and VP7 Proteins of Bluetongue Virus

    • Alejandro Marín-López, Javier Ortego
    Pages 137-150

  11. Generation of Recombinant Capripoxvirus Vectors for Vaccines and Gene Knockout Function Studies

    • Hani Boshra, Jingxin Cao, Shawn Babiuk
    Pages 151-161

  12. Recombinant Swinepox Virus for Veterinary Vaccine Development

    • Hong-Jie Fan, Hui-Xing Lin
    Pages 163-175

  13. Generation and Selection of Orf Virus (ORFV) Recombinants

    • Hanns-Joachim Rziha, Jörg Rohde, Ralf Amann
    Pages 177-200

  14. Polycistronic Herpesvirus Amplicon Vectors for Veterinary Vaccine Development

    • Anita Felicitas Meier, Andrea Sara Laimbacher, Mathias Ackermann
    Pages 201-224

  15. Construction and Application of Newcastle Disease Virus-Based Vector Vaccines

    • Paul J. Wichgers Schreur
    Pages 225-237

  16. Chimeric Pestivirus Experimental Vaccines

    • Ilona Reimann, Sandra Blome, Martin Beer
    Pages 239-246

  17. Analysis of the Cellular Immune Responses to Vaccines

    • Nicholas Svitek, Evans L. N. Taracha, Rosemary Saya, Elias Awino, Vishvanath Nene, Lucilla Steinaa
    Pages 247-262

  18. Back Matter

    Pages 263-265
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Keywords

About this book

This detailed volume explores the most popular antigen production and delivery strategies that have been tested in veterinary species. Viral vectors as well as genetic and protein subunit vaccines or large scale protein production systems are considered as well as an updated view of most options available for vaccine development, including the data obtained through experimental trials which contributes to the exploration and understanding of the immune mechanisms and immune correlates relevant in protection among different animal species. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols facilitates access to well-established protocols to those beginning in this interesting and laborious field as well as providing important basic knowledge when attempting a novel vaccine design or platform.

Editors and Affiliations

  • CISA-INIA, Valdeolmos, Spain

    Alejandro Brun

Bibliographic Information

  • Book Title: Vaccine Technologies for Veterinary Viral Diseases

  • Book Subtitle: Methods and Protocols

  • Editors: Alejandro Brun

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3008-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3007-4Published: 13 October 2015

  • Softcover ISBN: 978-1-4939-5000-3Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3008-1Published: 12 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 265

  • Topics: Immunology

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