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Whole Genome Amplification

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Thomas Kroneis

    1. Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Graz, Austria

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1347)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Principles of Whole-Genome Amplification

    • Zbigniew Tadeusz Czyz, Stefan Kirsch, Bernhard Polzer
    Pages 1-14

  3. Bias in Whole Genome Amplification: Causes and Considerations

    • Jeffrey Sabina, John H. Leamon
    Pages 15-41

  4. The Single-Cell Lab or How to Perform Single-Cell Molecular Analysis

    • Roland Kirchner, Marianna Alunni-Fabbroni
    Pages 43-55

  5. Sample Preparation Methods Following CellSearch Approach Compatible of Single-Cell Whole-Genome Amplification: An Overview

    • Joost F. Swennenhuis, Leon Terstappen
    Pages 57-67

  6. Deterministic Whole-Genome Amplification of Single Cells

    • Zbigniew Tadeusz Czyż, Christoph A. Klein
    Pages 69-86

  7. Construction of a DNA Library on Microbeads Using Whole Genome Amplification

    • Takaaki Kojima, Bo Zhu, Hideo Nakano
    Pages 87-100

  8. Heat-Induced Fragmentation and Adapter-Assisted Whole Genome Amplification Using GenomePlex® Single-Cell Whole Genome Amplification Kit (WGA4)

    • Amin El-Heliebi, Shukun Chen, Thomas Kroneis
    Pages 101-109

  9. Whole Genome Amplification by Isothermal Multiple Strand Displacement Using Phi29 DNA Polymerase

    • Thomas Kroneis, Amin El-Heliebi
    Pages 111-117

  10. Using Multiplex PCR for Assessing the Quality of Whole Genome Amplified DNA

    • Amin El-Heliebi, Shukun Chen, Thomas Kroneis
    Pages 119-128

  11. Quality Control of Isothermal Amplified DNA Based on Short Tandem Repeat Analysis

    • Thomas Kroneis, Amin El-Heliebi
    Pages 129-140

  12. Laser Microdissection of FFPE Tissue Areas and Subsequent Whole Genome Amplification by Ampli1™

    • Zbigniew Tadeusz Czyz, Nikolas H. Stoecklein, Bernhard Polzer
    Pages 141-162

  13. Whole Genome Amplification from Blood Spot Samples

    • Karina Meden Sørensen
    Pages 163-178

  14. Analysis of Whole Mitogenomes from Ancient Samples

    • Gloria Gonzales Fortes, Johanna L. A. Paijmans
    Pages 179-195

  15. Copy Number Variation Analysis by Array Analysis of Single Cells Following Whole Genome Amplification

    • Eftychia Dimitriadou, Masoud Zamani Esteki, Joris Robert Vermeesch
    Pages 197-219

  16. Whole Genome Amplification in Genomic Analysis of Single Circulating Tumor Cells

    • Christin Gasch, Klaus Pantel, Sabine Riethdorf
    Pages 221-232

  17. Whole Genome Amplification of Labeled Viable Single Cells Suited for Array-Comparative Genomic Hybridization

    • Thomas Kroneis, Amin El-Heliebi
    Pages 233-243

  18. Low-Volume On-Chip Single-Cell Whole Genome Amplification for Multiple Subsequent Analyses

    • Thomas Kroneis, Shukun Chen, Amin El-Heliebi
    Pages 245-261

  19. Detection and Characterization of Circulating Tumor Cells by the CellSearch Approach

    • Frank Coumans, Leon Terstappen
    Pages 263-278

  20. Back Matter

    Pages 279-284
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Keywords

About this book

This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and protocols that allow the assessment of WGA product quality. The final chapters contain advanced protocols and address issues such as sample preparation using laser-micro dissection; WGA from partially degraded DNA (formalin-fixed paraffin embedded samples); circulating tumor cells; and ancient samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Whole Genome Amplification: Methods and Protocols will serve as a rich source of detailed information and inspiration and will help researchers, both new and experienced, yield successful results.

Editors and Affiliations

  • Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Graz, Austria

    Thomas Kroneis

About the editor

DI Dr. Thomas Kroneis Medical University Graz Institute of Cell Biology, Histology & Embryology Head of Research Unit for Single Cell Analysis +43(0)316/380-4236 mailto: thomas.kroneis@medunigraz.at

Bibliographic Information

  • Book Title: Whole Genome Amplification

  • Book Subtitle: Methods and Protocols

  • Editors: Thomas Kroneis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2990-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2989-4Published: 16 September 2015

  • Softcover ISBN: 978-1-4939-4846-8Published: 22 October 2016

  • eBook ISBN: 978-1-4939-2990-0Published: 16 September 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 284

  • Number of Illustrations: 5 b/w illustrations, 13 illustrations in colour

  • Topics: Human Genetics

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