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  • © 2015

Immunosenescence

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1343)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
  2. Flow Cytometry Analysis of NK Cell Phenotype and Function in Aging

    • Raquel Tarazona, Carmen Campos, Alejandra Pera, Beatriz Sanchez-Correa, Rafael Solana
    Pages 9-18
  3. Flow Cytometric Identification of Fibrocytes in the Human Circulation

    • Xinyuan Hu, Erin M. DeBiasi, Erica L. Herzog
    Pages 19-33
  4. Experimental Approaches to Tissue Injury and Repair in Advanced Age

    • Aleah L. Brubaker, Stewart R. Carter, Elizabeth J. Kovacs
    Pages 35-51
  5. Multicolor Digital Flow Cytometry in Human Translational Immunology

    • Samit R. Joshi, Subhasis Mohanty, Albert C. Shaw
    Pages 53-64
  6. Flow Cytometry-Based Methods to Characterize Immune Senescence in Nonhuman Primates

    • Christine Meyer, Kristen Haberthur, Mark Asquith, Ilhem Messaoudi
    Pages 65-80
  7. Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry

    • Michael D. Leipold, Evan W. Newell, Holden T. Maecker
    Pages 81-95
  8. Imaging Immunosenescence

    • Feng Qian, Ruth R. Montgomery
    Pages 97-106
  9. Assays for Monitoring Macroautophagy Activity in T cells

    • Yair Botbol, Fernando Macian
    Pages 143-153
  10. Single-Cell Analysis of T-Cell Receptor αβ Repertoire

    • Pradyot Dash, George C. Wang, Paul G. Thomas
    Pages 181-197
  11. Assessment of B Cell Repertoire in Humans

    • Yu-Chang Wu, David Kipling, Deborah Dunn-Walters
    Pages 199-218
  12. Laboratory and Data Analysis Methods for Characterization of Human B Cell Repertoires by High-Throughput DNA Sequencing

    • Chen Wang, Yi Liu, Krishna M. Roskin, Katherine J. L. Jackson, Scott D. Boyd
    Pages 219-233
  13. Discovery of Novel microRNAs in Aging Caenorhabditis elegans

    • Alexandre de Lencastre, Frank Slack
    Pages 235-248
  14. Analysis of DNA Methylation by Pyrosequencing

    • Colin Delaney, Sanjay K. Garg, Raymond Yung
    Pages 249-264

About this book

In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire. In addition, Immunosenecence: Methods and Protocols guides readers through cell biology and biochemical methods for analyses of telomere dysfunction, autophagy, protein oxidation, microRNAs and DNA methylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Immunosenecence: Methods and Protocols provides a mixture of basic and advanced protocols that will be useful for immunologists in general and investigators in aging biology in particular.

Editors and Affiliations

  • Section of Infectious Diseases, Department of Internal Medicine, Yale School of Medicine, New Haven, USA

    Albert C. Shaw

Bibliographic Information

  • Book Title: Immunosenescence

  • Book Subtitle: Methods and Protocols

  • Editors: Albert C. Shaw

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2963-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media 2015

  • Hardcover ISBN: 978-1-4939-2962-7Published: 30 September 2015

  • Softcover ISBN: 978-1-4939-4418-7Published: 29 October 2016

  • eBook ISBN: 978-1-4939-2963-4Published: 29 September 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 270

  • Number of Illustrations: 22 b/w illustrations, 27 illustrations in colour

  • Topics: Immunology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access