Designer Receptors Exclusively Activated by Designer Drugs

1. Front Matter

   Pages i-xvii

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2. Defining the Functional Equivalence of Wild-Type and Chemically Engineered G Protein-Coupled Receptors

   • Elisa Alvarez-Curto, Graeme Milligan

   Pages 1-28

3. Design and Analysis of an Arrestin-Biased DREADD

   • Ken-ichiro Nakajima, Luis E. Diaz Gimenez, Vsevolod V. Gurevich, Jürgen Wess

   Pages 29-48

4. Regulation of Gene Transcription Following Stimulation of Gαq-Coupled Designer Receptors

   • Oliver G. Rössler, Gerald Thiel

   Pages 49-60

5. Chemogenetic Deconstruction of Feeding Circuits

   • Michael Krashes

   Pages 61-81

6. Dissecting Neuronal Circuits Involved in Olfactory-Mediated Behaviors

   • Alexia Nunez-Parra, Krista Krahe, Wilson Chan, Ricardo C. Araneda

   Pages 83-94

7. The Use of DREADDs (Designer Receptors Exclusively Activated by Designer Receptors) in Transgenic Mouse Behavioral Models

   • Daniela Cassataro, Lucas Sjulson

   Pages 95-108

8. Pharmacogenetic Dissection of Neural Mechanisms Underlying the Regulation of Sleep–Wakefulness Using DREADDs

   • Michihiro Mieda, Takeshi Sakurai

   Pages 109-127

9. DREADD’ed Addiction: Using Designer Receptors to Delineate Neural Circuits Underlying Drug-Seeking Behaviors

   • Sunila G. Nair, Denis Smirnov, John F. Neumaier

   Pages 129-145

10. DREADDs in Drosophila melanogaster

    • Charles D. Nichols, Jaime Becnel

    Pages 147-158

11. Back Matter

    Pages 159-160

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This volume provides current methods to analyze the properties of designer receptors exclusively activated by designer drugs (DREADDs) in vitro and to measure the biological responses of DREADD activation in different neuronal populations in vivo. Chapters focus on the utility of DREADD technology to selectively stimulate or inhibit neuronal activity in selected brain areas of transgenic mice. In addition, the successful use of DREADD expression in rats and Drosophila is described. Together, the DREADD technology represents a non-invasive, chemical-genetic tool for investigating neuronal signaling and the resulting behavioural responses in vivo. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory. Concise and easy-to-use, Designer Receptors Exclusively Activated by Designer Drugs aims to ensure successful results in the further study of this vital field.

• CNO-stimulated designer receptors
• DREADD
• G protein
• G-protein coupled receptors
• GPCR signaling
• designer drug
• designer receptors
• hM3Dq
• heterologous G proteins
• transgenic mice

• Department of Medical Biochemistry and Molecular Biology, University of Saarland Medical Faculty, Homburg, Germany

  Gerald Thiel

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