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Designer Receptors Exclusively Activated by Designer Drugs

  • Book
  • © 2015

Overview

Editors:
  1. Gerald Thiel

    1. Department of Medical Biochemistry and Molecular Biology, University of Saarland Medical Faculty, Homburg, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 108)

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Table of contents (9 protocols)

  1. Front Matter

    Pages i-xvii
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  2. Defining the Functional Equivalence of Wild-Type and Chemically Engineered G Protein-Coupled Receptors

    • Elisa Alvarez-Curto, Graeme Milligan
    Pages 1-28

  3. Design and Analysis of an Arrestin-Biased DREADD

    • Ken-ichiro Nakajima, Luis E. Diaz Gimenez, Vsevolod V. Gurevich, Jürgen Wess
    Pages 29-48

  4. Regulation of Gene Transcription Following Stimulation of Gαq-Coupled Designer Receptors

    • Oliver G. Rössler, Gerald Thiel
    Pages 49-60

  5. Chemogenetic Deconstruction of Feeding Circuits

    • Michael Krashes
    Pages 61-81

  6. Dissecting Neuronal Circuits Involved in Olfactory-Mediated Behaviors

    • Alexia Nunez-Parra, Krista Krahe, Wilson Chan, Ricardo C. Araneda
    Pages 83-94

  7. The Use of DREADDs (Designer Receptors Exclusively Activated by Designer Receptors) in Transgenic Mouse Behavioral Models

    • Daniela Cassataro, Lucas Sjulson
    Pages 95-108

  8. Pharmacogenetic Dissection of Neural Mechanisms Underlying the Regulation of Sleep–Wakefulness Using DREADDs

    • Michihiro Mieda, Takeshi Sakurai
    Pages 109-127

  9. DREADD’ed Addiction: Using Designer Receptors to Delineate Neural Circuits Underlying Drug-Seeking Behaviors

    • Sunila G. Nair, Denis Smirnov, John F. Neumaier
    Pages 129-145

  10. DREADDs in Drosophila melanogaster

    • Charles D. Nichols, Jaime Becnel
    Pages 147-158

  11. Back Matter

    Pages 159-160
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Keywords

About this book

This volume provides current methods to analyze the properties of designer receptors exclusively activated by designer drugs (DREADDs) in vitro and to measure the biological responses of DREADD activation in different neuronal populations in vivo. Chapters focus on the utility of DREADD technology to selectively stimulate or inhibit neuronal activity in selected brain areas of transgenic mice. In addition, the successful use of DREADD expression in rats and Drosophila is described. Together, the DREADD technology represents a non-invasive, chemical-genetic tool for investigating neuronal signaling and the resulting behavioural responses in vivo. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory. Concise and easy-to-use, Designer Receptors Exclusively Activated by Designer Drugs aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Medical Biochemistry and Molecular Biology, University of Saarland Medical Faculty, Homburg, Germany

    Gerald Thiel

Bibliographic Information

  • Book Title: Designer Receptors Exclusively Activated by Designer Drugs

  • Editors: Gerald Thiel

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-2944-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2943-6Published: 09 September 2015

  • Softcover ISBN: 978-1-4939-4957-1Published: 22 October 2016

  • eBook ISBN: 978-1-4939-2944-3Published: 04 September 2015

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XVII, 160

  • Number of Illustrations: 14 b/w illustrations, 14 illustrations in colour

  • Topics: Neurosciences

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