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Cartilage Tissue Engineering

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Pauline M. Doran

    1. Swinburne University of Technology, Faculty of Science, Engineering and Technology, Hawthorn, Melbourne, Australia

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1340)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Overview

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Cartilage Tissue Engineering: What Have We Learned in Practice?

      • Pauline M. Doran
      Pages 3-21

  3. Cell Isolation, Expansion, and Differentiation

    1. Front Matter

      Pages 23-23
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    2. Human Fetal and Adult Chondrocytes

      • Kifah Shahin, Nastaran Mahmoudifar, Pauline M. Doran
      Pages 25-40

    3. Mesenchymal Stem Cells Derived from Human Bone Marrow

      • Oliver F. W. Gardner, Mauro Alini, Martin J. Stoddart
      Pages 41-52

    4. Mesenchymal Stem Cells Derived from Human Adipose Tissue

      • Nastaran Mahmoudifar, Pauline M. Doran
      Pages 53-64

    5. Derivation and Chondrogenic Commitment of Human Embryonic Stem Cell-Derived Mesenchymal Progenitors

      • Hicham Drissi, Jason D. Gibson, Rosa M. Guzzo, Ren-He Xu
      Pages 65-78

    6. Differentiation of Human Induced Pluripotent Stem Cells to Chondrocytes

      • Rosa M. Guzzo, Hicham Drissi
      Pages 79-95

    7. Gene Transfer and Gene Silencing in Stem Cells to Promote Chondrogenesis

      • Feng Zhang, Dong-An Wang
      Pages 97-117

  4. Biomaterials and Scaffolds

    1. Front Matter

      Pages 119-119
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    2. Hydrogels with Tunable Properties

      • Peggy P. Y. Chan
      Pages 121-132

    3. Decellularized Extracellular Matrix Scaffolds for Cartilage Regeneration

      • Shraddha Thakkar, Hugo Fernandes, Lorenzo Moroni
      Pages 133-151

    4. Use of Interim Scaffolding and Neotissue Development to Produce a Scaffold-Free Living Hyaline Cartilage Graft

      • Ting Ting Lau, Wenyan Leong, Yvonne Peck, Kai Su, Dong-An Wang
      Pages 153-160

    5. Bioprinted Scaffolds for Cartilage Tissue Engineering

      • Hyun-Wook Kang, James J. Yoo, Anthony Atala
      Pages 161-169

    6. Scaffolds for Controlled Release of Cartilage Growth Factors

      • Marie Morille, Marie-Claire Venier-Julienne, Claudia N. Montero-Menei
      Pages 171-180

    7. Nanostructured Capsules for Cartilage Tissue Engineering

      • Clara R. Correia, Rui L. Reis, João F. Mano
      Pages 181-189

    8. Stratified Scaffolds for Osteochondral Tissue Engineering

      • Patcharakamon Nooeaid, Gundula Schulze-Tanzil, Aldo R. Boccaccini
      Pages 191-200

  5. Bioreactors

    1. Front Matter

      Pages 201-201
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    2. Mechanobioreactors for Cartilage Tissue Engineering

      • Joanna F. Weber, Roman Perez, Stephen D. Waldman
      Pages 203-219
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Keywords

About this book

This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffolds; design and operation of bioreactors; in vivo testing of engineered constructs; and molecular and functional analysis of cartilage cells and tissues. Frequently used technologies are covered, as well as more recent advances in inspirational areas such as ‘smart’ biomaterial development, novel bioreactor design, -omics analysis, and genetic manipulation of matrix synthesis. The key procedures discussed either underpin the progress already achieved in cartilage tissue engineering or are indicative of the direction of future research in the area. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cartilage Tissue Engineering: Methods and Protocols is a useful resource that informs the scientific community about the experimental work covering a broad range of objectives for cartilage synthesis and regeneration.

Editors and Affiliations

  • Swinburne University of Technology, Faculty of Science, Engineering and Technology, Hawthorn, Melbourne, Australia

    Pauline M. Doran

About the editor

Pauline M. Doran Professor Faculty of Life and Social Sciences Swinburne University of Technology PO Box 218 Hawthorn VIC 3122 Australia Ph: +61-3-9214-5716 Email: pdoran@swin.edu.au

Bibliographic Information

  • Book Title: Cartilage Tissue Engineering

  • Book Subtitle: Methods and Protocols

  • Editors: Pauline M. Doran

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2938-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2937-5Published: 09 October 2015

  • Softcover ISBN: 978-1-4939-4897-0Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2938-2Published: 07 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 290

  • Number of Illustrations: 9 b/w illustrations, 38 illustrations in colour

  • Topics: Biotechnology, Regenerative Medicine/Tissue Engineering

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