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Cyclin-Dependent Kinase (CDK) Inhibitors

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Mar Orzáez

    1. Medicinal Chemistry, Centro de Investigación Príncipe Felipe, Valencia, Spain

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    You can also search for this editor in PubMed   Google Scholar

  2. Mónica Sancho Medina

    1. Medicinal Chemistry, Centro de Investigación Príncipe Felipe, Valencia, Spain

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    You can also search for this editor in PubMed   Google Scholar

  3. Enrique Pérez-Payá

    1. IBV-CSIC and Centro de Investigación Príncipe Felipe, Valencia, Spain

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1336)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Immunoprecipitation of Cdk–Cyclin Complexes for Determination of Kinase Activity

    • Edurne Gallastegui, Oriol Bachs
    Pages 1-8

  3. Expression and Purification of Recombinant Cyclins and CDKs for Activity Evaluation

    • Edurne Gallastegui, Oriol Bachs
    Pages 9-12

  4. Expression and Purification of Recombinant CDKs: CDK7, CDK8, and CDK9

    • Reena Pinhero, Krassimir Yankulov
    Pages 13-28

  5. Preparation of CDK/Cyclin Inhibitor Complexes for Structural Determination

    • Asterios I. Grigoroudis, George Kontopidis
    Pages 29-45

  6. Fragment-Based De Novo Design of Cyclin-Dependent Kinase 2 Inhibitors

    • Sunil Kumar Tripathi, Poonam Singh, Sanjeev Kumar Singh
    Pages 47-58

  7. Protein-Protein Interaction for the De Novo Design of Cyclin-Dependent Kinase Peptide Inhibitors

    • Karthiga Arumugasamy, Sunil Kumar Tripathi, Poonam Singh, Sanjeev Kumar Singh
    Pages 59-66

  8. Identification of Cyclin A Binders with a Fluorescent Peptide Sensor

    • Elena Pazos, José L. Mascareñas, M. Eugenio Vázquez
    Pages 67-83

  9. Cell Synchronization Techniques to Study the Action of CDK Inhibitors

    • Beatriz Pérez-Benavente, Rosa Farràs
    Pages 85-93

  10. Analysis of CDK Inhibitor Action on Mitochondria-Mediated Apoptosis

    • Anna Gortat
    Pages 95-109

  11. Evaluating the Effects of CDK Inhibitors in Ischemia–Reperfusion Injury Models

    • Tatiana Guevara
    Pages 111-121

  12. Assessing Cell Cycle Independent Function of the CDK Inhibitor p21CDKN1A in DNA Repair

    • Ilaria Dutto, Micol Tillhon, Ennio Prosperi
    Pages 123-139

  13. Drug Delivery Strategies of Chemical CDK Inhibitors

    • Daniel Alvira, Laura Mondragón
    Pages 141-154

  14. Animal Models for Studying the In Vivo Functions of Cell Cycle CDKs

    • Sanjiv Risal, Deepak Adhikari, Kui Liu
    Pages 155-166

  15. Evaluating Chemical CDK Inhibitors as Cell Death Inducers

    • Hiroshi Hirai, Yoko Nakatsuru
    Pages 167-178

  16. Models for the Study of the Cross Talk Between Inflammation and Cell Cycle

    • Laura J. Hoodless, Calum T. Robb, Jennifer M. Felton, Carl S. Tucker, Adriano G. Rossi
    Pages 179-209

  17. Metabolomic Applications to the Characterization of the Mode-of-Action of CDK Inhibitors

    • Martina Palomino-Schätzlein, Antonio Pineda-Lucena
    Pages 211-223

  18. Back Matter

    Pages 225-227
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Keywords

About this book

This volume contains a collection of relevant information for drug discovery in cell cycle research. Protocols to develop screening assays or to identify novel CDK inhibitors are discussed in the first part of the book. The second part of the book describes elaborate procedures to evaluate activity and mechanism of action of new and already identified CDK inhibitors. The third part of the book talks about protocols to evaluate metabolomics changes associated to inhibitor treatment. Drug delivery strategies focused on nanoparticle development to provide alternative internalization systems for increasing inhibitor efficacy are also described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Cyclin-Dependent Kinase (CDK) Inhibitors: Methods and Protocols is a useful tool for scientists interested in this research field.

Editors and Affiliations

  • Medicinal Chemistry, Centro de Investigación Príncipe Felipe, Valencia, Spain

    Mar Orzáez, Mónica Sancho Medina

  • IBV-CSIC and Centro de Investigación Príncipe Felipe, Valencia, Spain

    Enrique Pérez-Payá

Bibliographic Information

  • Book Title: Cyclin-Dependent Kinase (CDK) Inhibitors

  • Book Subtitle: Methods and Protocols

  • Editors: Mar Orzáez, Mónica Sancho Medina, Enrique Pérez-Payá

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2926-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2016

  • Hardcover ISBN: 978-1-4939-2925-2Published: 05 August 2015

  • Softcover ISBN: 978-1-4939-4368-5Published: 19 October 2016

  • eBook ISBN: 978-1-4939-2926-9Published: 31 July 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 227

  • Number of Illustrations: 20 b/w illustrations, 22 illustrations in colour

  • Topics: Pharmacology/Toxicology

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