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  • © 2015

Carbohydrate-Based Vaccines

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1331)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-xi
  2. Carbohydrate-Based Vaccines: An Overview

    • Julia Hütter, Bernd Lepenies
    Pages 1-10
  3. Glycans as Vaccine Antigens and Adjuvants: Immunological Considerations

    • Stephanie Zimmermann, Bernd Lepenies
    Pages 11-26
  4. The Glycan Array Platform as a Tool to Identify Carbohydrate Antigens

    • Li Xia, Jeffrey C. Gildersleeve
    Pages 27-40
  5. Antibody-Carbohydrate Recognition from Docked Ensembles Using the AutoMap Procedure

    • Tamir Dingjan, Mark Agostino, Paul A. Ramsland, Elizabeth Yuriev
    Pages 41-55
  6. Generation of Monoclonal Antibodies against Defined Oligosaccharide Antigens

    • Felix Broecker, Chakkumkal Anish, Peter H. Seeberger
    Pages 57-80
  7. Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility

    • David Bitto, David J. Harvey, Steinar Halldorsson, Katie J. Doores, Laura K. Pritchard, Juha T. Huiskonen et al.
    Pages 93-121
  8. N-Glycosylation Fingerprinting of Viral Glycoproteins by xCGE-LIF

    • René Hennig, Erdmann Rapp, Robert Kottler, Samanta Cajic, Matthias Borowiak, Udo Reichl
    Pages 123-143
  9. Gold Nanoparticles as Carriers for Synthetic Glycoconjugate Vaccines

    • Fabrizio Chiodo, Marco Marradi
    Pages 159-171
  10. Identification and Characterization of Carbohydrate-Based Adjuvants

    • Timo Johannssen, Bernd Lepenies
    Pages 173-187
  11. Characterization of Carbohydrate Vaccines by NMR Spectroscopy

    • Francesco Berti, Neil Ravenscroft
    Pages 189-209
  12. Characterization of Capsular Polysaccharides and Their Glycoconjugates by Hydrodynamic Methods

    • Stephen E. Harding, Ali Saber Abdelhameed, Richard B. Gillis, Gordon A. Morris, Gary G. Adams
    Pages 211-227
  13. Glycoconjugate Vaccines: The Regulatory Framework

    • Christopher Jones
    Pages 229-251
  14. Back Matter

    Pages 253-255

About this book

This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Carbohydrate-Based Vaccines: Methods and Protocols aims to be a valuable manual that will facilitate research in the field of carbohydrate-based vaccines.

Editors and Affiliations

  • Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine, Hannover, Germany

    Bernd Lepenies

Bibliographic Information

  • Book Title: Carbohydrate-Based Vaccines

  • Book Subtitle: Methods and Protocols

  • Editors: Bernd Lepenies

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2874-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2873-6Published: 14 July 2015

  • Softcover ISBN: 978-1-4939-4919-9Published: 15 October 2016

  • eBook ISBN: 978-1-4939-2874-3Published: 13 July 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 255

  • Number of Illustrations: 52 b/w illustrations, 21 illustrations in colour

  • Topics: Immunology

Buy it now

Buying options

eBook USD 69.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 89.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 119.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access