Muscarinic Receptor: From Structure to Animal Models

1. Front Matter

   Pages i-xii

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2. Towards the Crystal Structure Determination of Muscarinic Acetylcholine Receptors

   • Ryoji Suno, Hidetsugu Asada, Takuya Kobayashi

   Pages 1-13

3. Homology Modeling and Docking Evaluation of Human Muscarinic Acetylcholine Receptors

   • Trayder Thomas, David K. Chalmers, Elizabeth Yuriev

   Pages 15-35

4. Radioligand Binding at Muscarinic Receptors

   • Esam E. El-Fakahany, Jan Jakubik

   Pages 37-68

5. Binding Method for Detection of Muscarinic Acetylcholine Receptors in Receptor’s Natural Environment

   • Ikunobu Muramatsu, Hatsumi Yoshiki, Kiyonao Sada, Junsuke Uwada, Takanobu Taniguchi, Takayoshi Masuoka et al.

   Pages 69-81

6. Use of Antibodies in the Research on Muscarinic Receptor Subtypes

   • Wisuit Pradidarcheep, Martin C. Michel

   Pages 83-94

7. Allosteric Modulation of Muscarinic Receptors

   • Jan Jakubik, Esam E. El-Fakahany

   Pages 95-130

8. Subcellular and Synaptic Localization of Muscarinic Receptors in Neurons Using High-Resolution Electron Microscopic Preembedding Immunogold Technique

   • Véronique Bernard

   Pages 131-146

9. Investigation of Muscarinic Receptors by Fluorescent Techniques

   • Cornelius Krasel, Andreas Rinne, Moritz Bünemann

   Pages 147-158

10. Autoradiography Assessment of Muscarinic Receptors in the Central Nervous System

    • Vladimir Farar, Jaromir Myslivecek

    Pages 159-180

11. Imaging of Muscarinic Receptors in the Central Nervous System

    • Hideo Tsukada, Shingo Nishiyama, Kazuhiro Takahashi

    Pages 181-203

12. Detection of Non-neuronal Acetylcholine

    • Ignaz Karl Wessler, Charles James Kirkpatrick

    Pages 205-220

13. Utilization of Superfused Cerebral Slices in Probing Muscarinic Receptor Autoregulation of Acetylcholine Release

    • Glenda Alquicer, Vladimír Doležal, Esam E. El-Fakahany

    Pages 221-233

14. Regulation of Heart Contractility by M2 and M3 Muscarinic Receptors: Functional Studies Using Muscarinic Receptor Knockout Mouse

    • Takio Kitazawa, Hiroki Teraoka, Nao Harada, Kenta Ochi, Tatsuro Nakamura, Koichi Asakawa et al.

    Pages 235-259

15. Muscarinic Receptor Gene Transfections and In Vivo Dopamine Electrochemistry: Muscarinic Receptor Control of Dopamine-Dependent Reward and Locomotion

    • Stephan Steidl, David Ian Wasserman, Charles D. Blaha, John Yeomans

    Pages 261-282

16. Back Matter

    Pages 283-287

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This volume provides methods for characterization of muscarinic receptor in crystallography studies that advanced our understanding of structural properties and activation mechanism of muscarinic receptors and are cornerstone in molecular modeling and computer‑based approaches to study muscarinic receptors. Muscarinic Receptor: From Structure to Animal Models guides readers through binding techniques, protocols to investigate molecular properties of muscarinic receptors, protocols to study muscarinic receptors in the central nervous system using autoradiography and PET studies and protocols on animals with knock-out and knock-in muscarinic genes to study role of muscarinic receptors in physiology and behavior. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Concise and easy-to-use, Muscarinic Receptor: From Structure to Animal Models aims to ensure successful results in the further study of this vital field.

• PET studies
• autoradiography
• central nervous system
• crystallography
• heterologous expression systems
• knock-out& knock-in muscarinic genes
• ligands
• molecular modeling
• subtype selective ligands
• systemic level

• Institute of Physiology, Charles University, Prague, Czech Republic

  Jaromir Myslivecek

• Department of Neurochemistry, Acad. of Sciences of the Czech Rep. Institute of Physiology, Prague, Czech Republic

  Jan Jakubik

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