Bacterial Persistence

1. Front Matter

   Pages i-xi

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2. Introduction

   1. Front Matter

      Pages 1-1

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   2. A Historical Perspective on Bacterial Persistence

      • Natalie Verstraeten, Wouter Knapen, Maarten Fauvart, Jan Michiels

      Pages 3-13

3. Quantification of Persistence

   1. Front Matter

      Pages 15-15

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   2. Persisters: Methods for Isolation and Identifying Contributing Factors—A Review

      • Sarah E. Rowe, Brian P. Conlon, Iris Keren, Kim Lewis

      Pages 17-28

   3. A General Method for Measuring Persister Levels in Escherichia coli Cultures

      • Niilo Kaldalu, Arvi Jõers, Henri Ingelman, Tanel Tenson

      Pages 29-42

   4. Optimized Method for Measuring Persistence in Escherichia coli with Improved Reproducibility

      • F. Goormaghtigh, L. Van Melderen

      Pages 43-52

   5. A Microplate-Based System as In Vitro Model of Biofilm Growth and Quantification

      • Ilse Vandecandelaere, Heleen Van Acker, Tom Coenye

      Pages 53-66

   6. Protocol for Determination of the Persister Subpopulation in Candida Albicans Biofilms

      • Katrijn De Brucker, Kaat De Cremer, Bruno P. A. Cammue, Karin Thevissen

      Pages 67-72

4. Single Cell Analysis of Persister Cells

   1. Front Matter

      Pages 73-73

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   2. Quantitative Measurements of Type I and Type II Persisters Using ScanLag

      • Irit Levin-Reisman, Nathalie Q. Balaban

      Pages 75-81

   3. Analyzing Persister Physiology with Fluorescence-Activated Cell Sorting

      • Mehmet A. Orman, Theresa C. Henry, Christina J. DeCoste, Mark P. Brynildsen

      Pages 83-100

   4. Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array

      • Ryota Iino, Shouichi Sakakihara, Yoshimi Matsumoto, Kunihiko Nishino

      Pages 101-109

5. Identification of Persister Mutants and Genes

   1. Front Matter

      Pages 111-111

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   2. A Whole-Cell-Based High-Throughput Screening Method to Identify Molecules Targeting Pseudomonas aeruginosa Persister Cells

      • Veerle Liebens, Valerie Defraine, Maarten Fauvart

      Pages 113-120

   3. Functional Analysis of the Role of Toxin–Antitoxin (TA) Loci in Bacterial Persistence

      • Aaron T. Butt, Richard W. Titball

      Pages 121-129

   4. Experimental Evolution of Escherichia coli Persister Levels Using Cyclic Antibiotic Treatments

      • Bram Van den Bergh, Joran E. Michiels, Jan Michiels

      Pages 131-143

6. Cellular and Animal Model Systems for Studying Persistence

   1. Front Matter

      Pages 145-145

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   2. In Vitro Models for the Study of the Intracellular Activity of Antibiotics

      • Julien M. Buyck, Sandrine Lemaire, Cristina Seral, Ahalieyah Anantharajah, Frédéric Peyrusson, Paul M. Tulkens et al.

      Pages 147-157

   3. A Murine Model for Escherichia coli Urinary Tract Infection

      • Thomas J. Hannan, David A. Hunstad

      Pages 159-175

This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in Escherichia coli cultures, protocols for the determination of the persister subpopulation in Candida albicans, quantitative measurements of Type I and Type II persisters using ScanLag, to in vitro and in vivo models for the study of the intracellular activity of antibiotics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols brings together the most respected researchers in bacterial persistence whose studies will remain vital to understanding this field for many years to come.

• Antibiotics
• Bacteria
• Mycobacterium Tuberculosis
• Persister Cells
• Pseudomonas Aeruginosa
• Staphylococcus Aureus
• antibacterial drug resistance

• Department of Microbial and Molecular Systems, KU Leuven - University of Leuven, Heverlee, Belgium

  Jan Michiels, Maarten Fauvart

Jan Michiels Centre of Microbial and Plant Genetics Department of Microbial and Molecular Systems, KU Leuven - University of Leuven Kasteelpark Arenberg 20 box 2460, B-3001 Heverlee Phone: +32 16 32 16 31 (secretariat) or +32 16 32 96 84 (office) Fax.: +32 16 32 19 63 Email: jan.michiels@biw.kuleuven.be Maarten Fauvart Centre of Microbial and Plant Genetics Department of Microbial and Molecular Systems, KU Leuven - University of Leuven Kasteelpark Arenberg 20 box 2460, B-3001 Heverlee Tel: +32 16 32 16 31 (secretariat) or +32 16 32 96 80 (office); Fax: +32 16 32 19 63 Email: maarten.fauvart@biw.kuleuven.be

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