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Cell Reprogramming

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Paul J. Verma

    1. Stem Cell and Genetic Engineering Group, Department of Materials Engineering Faculty of Engineering, Monash University, Clayton, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Huseyin Sumer

    1. Swinburne University of Technology, Hawthorn, Australia

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1330)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  4. Livestock, Domestic and Endangered Species

    1. Front Matter

      Pages 55-55
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    2. Inducing Pluripotency in Cattle

      • Luis F. Malaver-Ortega, Amir Taheri-Ghahfarokhi, Huseyin Sumer
      Pages 57-68

    3. Generation of Induced Pluripotent Stem Cells (iPSCs) from Adult Canine Fibroblasts

      • Sehwon Koh, Jorge A. Piedrahita
      Pages 69-78

    4. Derivation of Equine-Induced Pluripotent Stem Cell Lines Using a piggyBac Transposon Delivery System and Temporal Control of Transgene Expression

      • Kristina Nagy, Andras Nagy
      Pages 79-88

    5. Generation of Avian Induced Pluripotent Stem Cells

      • Yangqing Lu, Franklin D. West, Brian J. Jordan, Robert B. Beckstead, Erin T. Jordan, Steven L. Stice
      Pages 89-99

    6. Generation of Induced Pluripotent Stem Cells from Mammalian Endangered Species

      • Inbar Friedrich Ben-Nun, Susanne C. Montague, Marlys L. Houck, Oliver Ryder, Jeanne F. Loring
      Pages 101-109

  5. Germ-Line Transmission of Pluripotent Stem Cells

    1. Front Matter

      Pages 111-111
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    2. Generation of Efficient Germ-Line Chimeras Using Embryonic Stem Cell Injection

      • William A. Ritchie
      Pages 113-123

    3. Generation of Viable Mice from Induced Pluripotent Stem Cells (iPSCs) Through Tetraploid Complementation

      • Lan Kang, Shaorong Gao
      Pages 125-132

    4. Cloning Endangered Felids by Interspecies Somatic Cell Nuclear Transfer

      • Martha C. Gómez, C. Earle Pope
      Pages 133-152

    5. Generation of Chimeras from Porcine Induced Pluripotent Stem Cells

      • Franklin D. West, Steve L. Terlouw, John R. Dobrinsky, Yangqing Lu, Erin T. Jordan, Steven L. Stice
      Pages 153-167

    6. A Novel Method of Somatic Cell Nuclear Transfer with Minimum Equipment

      • S. M. Hosseini, F. Moulavi, M. H. Nasr-Esfahani
      Pages 169-188
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Keywords

About this book

This volume provides an understanding of the factors involved in nuclear reprogramming, which is essential for the success of reprogramming. The book is aimed at reprogramming differentiated cells and germ line transmission of pluripotent stem cells and features chapters that deal with reprogramming-related issues such as analysis of mitochondrial DNA in reprogrammed cells and the isolation of reprogramming intermediates; alternative methods for nuclear transfer; the production of germ-line chimeras from embryonic stem cells and induced pluripotent stem cells; and neonatal care and management of somatic cell nuclear transfer derived offspring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, is the perfect guide for molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians and animal care technicians involved with reprogramming, nuclear transfer and transgenesis.

Editors and Affiliations

  • Stem Cell and Genetic Engineering Group, Department of Materials Engineering Faculty of Engineering, Monash University, Clayton, Australia

    Paul J. Verma

  • Swinburne University of Technology, Hawthorn, Australia

    Huseyin Sumer

About the editors

Professor Paul J. Verma PhD Principal Scientist- Reproductive Biology South Australian Research Institute (SARDI) Adjunct Professor & Head Cell Reprogramming & Stem Cells Monash Institute of Medical Research 27-31 Wright Street Clayton, VIC 3168 AUSTRALIA Voice: + 61 3 9902 4771 Fax: + 61 3 9594 7416 Cell: +61 413 387 563 Email: Paul.Verma@sa.gov.au Email: Paul.Verma@monash.edu Huseyin Sumer Monash Institute of Medical Research Faculty of Medicine, Nursing and Health Sciences MIMR Centre for Reproduction and Development Phone: 61 2 990 24739 email: huseyin.sumer@monash.edu

Bibliographic Information

  • Book Title: Cell Reprogramming

  • Book Subtitle: Methods and Protocols

  • Editors: Paul J. Verma, Huseyin Sumer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2848-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2847-7Published: 02 December 2015

  • Softcover ISBN: 978-1-4939-7975-2Published: 13 March 2019

  • eBook ISBN: 978-1-4939-2848-4Published: 30 November 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 271

  • Number of Illustrations: 7 b/w illustrations, 20 illustrations in colour

  • Topics: Cell Biology

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