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Malaria Vaccines

Methods and Protocols

  • Book
  • © 2015

Overview

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1325)

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Table of contents (22 protocols)

  1. Pre-erythrocytic Stages

  2. Mosquito Stages

Keywords

About this book

This volume focuses on malaria vaccinology, and introduces researchers to a complete overview of the protocols and tools used by molecular and cellular malariologists. The chapters within this book talk about interventions concerning all aspects of life cycle progression--measuring antibody responses to blood stage parasite survival, the T Cell responses engendered by attenuated sporozoite vaccinations, and the unique effect on transmission of antibodies that target the mosquito stage of the life cycle. Additionally, methods concerning the ability to generate targeted gene deletions and replacements in the genome of Plasmodium parasites convey how Plasmodium parasite phenotypes can be created to precise specifications. More recently, the potential power of humanized mouse models of disease progression have been demonstrated and are also discussed in the book. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Malaria Vaccines: Methods and Protocols is a useful and unique resource for any aspiring malaria vaccinologists because of the volume's exquisitely detailed methodologies that are not typically found in published literature.

Editors and Affiliations

  • Center for Infectious Disease Research, Seattle, USA

    Ashley Vaughan

About the editor

Ashley Vaughan, Ph.D. Senior Scientist Seattle Biomedical Research Institute (Seattle BioMed) │307 Westlake Avenue N, Suite 500│Seattle, WA 98109 Phone: (206) 256-7115│ E-mail: ashley.vaughan@seattlebiomed.org

Bibliographic Information

  • Book Title: Malaria Vaccines

  • Book Subtitle: Methods and Protocols

  • Editors: Ashley Vaughan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2815-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2814-9Published: 10 October 2015

  • Softcover ISBN: 978-1-4939-4902-1Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2815-6Published: 08 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 305

  • Number of Illustrations: 7 b/w illustrations, 30 illustrations in colour

  • Topics: Immunology, Vaccine

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