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Ionotropic Glutamate Receptor Technologies

  • Book
  • © 2016

Overview

Editors:
  1. Gabriela K. Popescu

    1. Department of Biochemistry, University at Buffalo, Buffalo, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 106)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
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  2. Protein Assembly and Trafficking

    1. Front Matter

      Pages 1-1
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    2. Assaying AMPA Receptor Oligomerization

      • Catherine L. Salussolia, Quan Gan, Lonnie P. Wollmuth
      Pages 3-14

    3. A Step-by-Step Guide to Single-Subunit Counting of Membrane-Bound Proteins in Mammalian Cells

      • Mark R. P. Aurousseau, Hugo McGuire, Rikard Blunck, Derek Bowie
      Pages 15-30

    4. Counting NMDA Receptors at the Cell Surface

      • Martin Horak, Young Ho Suh
      Pages 31-44

    5. Electrophysiological Tagging of Ionotropic Glutamate Receptors

      • Andres Barria
      Pages 45-60

    6. Electron Microscopy Analysis of AMPA Receptors in Dendritic Spines

      • Audra A. Kramer, Amber N. Petersen, Nashaat Z. Gerges
      Pages 61-69

    7. Functional Detection of Novel Triheteromeric NMDA Receptors

      • Sanjay S. Kumar
      Pages 71-80

  3. Protein Structure and Dynamics

    1. Front Matter

      Pages 81-81
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    2. Expression, Purification, and Crystallization of Full Length Ionotropic Glutamate Receptors

      • Maria V. Yelshanskaya, Kei Saotome, Minfen Li, Alexander I. Sobolevsky
      Pages 83-100

    3. NMR Approaches to Functional Dynamics of Genetically Separated iGluR Domains

      • Christopher P. Ptak, Ahmed H. Ahmed, Robert E. Oswald
      Pages 101-118

    4. Computing Conformational Free Energies of iGluR Ligand-Binding Domains

      • Alvin Yu, Tyler Wied, John Belcher, Albert Y. Lau
      Pages 119-132

    5. LRET Methods for Investigating Conformational Changes in Functional Ionotropic Glutamate Receptors

      • Rita E. Sirrieh, Vasanthi Jayaraman
      Pages 133-144

    6. Assaying the Energetics of NMDA Receptor Pore Opening

      • Rashek Kazi, Melissa Daniel, Lonnie P. Wollmuth
      Pages 145-162

  4. Protein Function

    1. Front Matter

      Pages 163-163
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    2. Constructing a Rapid Solution Exchange System

      • David M. MacLean
      Pages 165-183

    3. Assessing The Effects of Ligand-Binding Mutations to AMPA and Kainate Receptor Kinetics

      • Mark W. Fleck
      Pages 185-203

    4. Analysis of Whole-Cell NMDA Receptor Currents

      • Vojtech Vyklicky, Miloslav Korinek, Ales Balik, Tereza Smejkalova, Barbora Krausova, Ladislav Vyklicky
      Pages 205-219

    5. Calcium Imaging to Study NMDA Receptor-mediated Cellular Responses

      • Kelly A. Krogh, Stanley A. Thayer
      Pages 221-239

    6. Timing AMPA Receptor Activation with Laser-Pulse Photolysis

      • Li Niu
      Pages 241-255
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Keywords

About this book

This detailed volume explores key technologies that are used currently to investigate iGluR structure, function and physiology.  Chapters in this book cover methods to help illuminate the assembly, trafficking, molecular composition and subcellular location of iGluRs; approaches used to understand the atomic organization of iGluRs; and techniques to monitor receptor activity in real time. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Concise and easy-to-use, Ionotropic Glutamate Receptor Technologies aims to facilitate the implementation of specific methods to iGluR investigations.

Editors and Affiliations

  • Department of Biochemistry, University at Buffalo, Buffalo, USA

    Gabriela K. Popescu

Bibliographic Information

  • Book Title: Ionotropic Glutamate Receptor Technologies

  • Editors: Gabriela K. Popescu

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-2812-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-2811-8Published: 26 September 2015

  • Softcover ISBN: 978-1-4939-4895-6Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2812-5Published: 25 September 2015

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XII, 304

  • Number of Illustrations: 23 b/w illustrations, 47 illustrations in colour

  • Topics: Neurosciences, Human Physiology

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