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Glyco-Engineering

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Alexandra Castilho

    1. University of Natural Resources and Life Sciences, Vienna, Austria

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  • Features protocols in glyco-engineering in bacteria, yeast, insects, plants and mammalian cells
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1321)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xvi
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  2. Glyco-Engineering in Bacteria

    1. Front Matter

      Pages 1-1
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    2. Current Approaches to Engineering N-Linked Protein Glycosylation in Bacteria

      • Andreas Naegeli, Markus Aebi
      Pages 3-16

    3. Inverse Metabolic Engineering for Enhanced Glycoprotein Production in Escherichia coli

      • Stephen R. P. Jaffé, Benjamin Strutton, Jagroop Pandhal, Phillip C. Wright
      Pages 17-35

    4. GlycoSNAP: A High-Throughput Screening Methodology for Engineering Designer Glycosylation Enzymes

      • Anne A. Ollis, Yi Chai, Matthew P. DeLisa
      Pages 37-47

    5. Production of Glycoproteins with Asparagine-Linked N-Acetylglucosamine in Escherichia coli

      • Flavio Schwarz, Markus Aebi
      Pages 49-56

    6. Glyco-engineering O-Antigen-Based Vaccines and Diagnostics in E. coli

      • Nicolas F. Vozza, Mario F. Feldman
      Pages 57-70

  3. Glyco-Engineering in Yeast

    1. Front Matter

      Pages 71-71
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    2. Progress in Yeast Glycosylation Engineering

      • Stephen R. Hamilton, Dongxing Zha
      Pages 73-90

    3. Protein Production with a Pichia pastoris OCH1 Knockout Strain in Fed-Batch Mode

      • Christoph Gmeiner, Oliver Spadiut
      Pages 91-101

    4. Engineering the Pichia pastoris N-Glycosylation Pathway Using the GlycoSwitch Technology

      • Bram Laukens, Charlot De Wachter, Nico Callewaert
      Pages 103-122

    5. Development of a Valuable Yeast Strain Using a Novel Mutagenesis Technique for the Effective Production of Therapeutic Glycoproteins

      • Hiroko Abe, Ken-ichi Nakayama
      Pages 123-127

  4. Glyco-Engineering in Insects

    1. Front Matter

      Pages 129-129
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    2. An Overview and History of Glyco-Engineering in Insect Expression Systems

      • Christoph Geisler, Hideaki Mabashi-Asazuma, Donald L. Jarvis
      Pages 131-152

    3. SweetBac: Applying MultiBac Technology Towards Flexible Modification of Insect Cell Glycosylation

      • Dieter Palmberger, Dubravko Rendic
      Pages 153-169

    4. Engineering Sialic Acid Synthesis Ability in Insect Cells

      • Karthik Viswanathan, Someet Narang, Michael J. Betenbaugh
      Pages 171-178

    5. Engineering N-Glycosylation Pathway in Insect Cells: Suppression of β-N-Acetylglucosaminidase and Expression of β-1,4-Galactosyltransferase

      • Yeon Kyu Kim, Hyung Joon Cha
      Pages 179-191

  5. Glyco-Engineering in Plants

    1. Front Matter

      Pages 193-193
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    2. N-Glyco-Engineering in Plants: Update on Strategies and Major Achievements

      • Herta Steinkellner, Alexandra Castilho
      Pages 195-212

    3. Gene Targeting for Precision Glyco-Engineering: Production of Biopharmaceuticals Devoid of Plant-Typical Glycosylation in Moss Bioreactors

      • Eva L. Decker, Gertrud Wiedemann, Ralf Reski
      Pages 213-224
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Keywords

About this book

Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself. The first five sections deal with host-specific glyco-engineering and contain chapters that provide protocols for modifications of the glycosylation pathway in bacteria, yeast, insect, plants and mammalian cells, while the last two sections explore alternative approaches to host glyco-engineering and selected protocols for the analysis of the N-glycans and glyco-profiling by mass spectrometry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and extensive, Glyco-Engineering: Methods and Protocols offers vast options to help researchers to choose the expression system and approach that best suits their intended protein research or applications.

Editors and Affiliations

  • University of Natural Resources and Life Sciences, Vienna, Austria

    Alexandra Castilho

Bibliographic Information

  • Book Title: Glyco-Engineering

  • Book Subtitle: Methods and Protocols

  • Editors: Alexandra Castilho

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2760-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2759-3Published: 17 June 2015

  • Softcover ISBN: 978-1-4939-4942-7Published: 09 October 2016

  • eBook ISBN: 978-1-4939-2760-9Published: 16 June 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 439

  • Number of Illustrations: 27 b/w illustrations, 52 illustrations in colour

  • Topics: Protein Science

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