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Yeast Surface Display

Methods, Protocols, and Applications

  • Book
  • © 2015

Overview

Editors:
  1. Bin Liu

    1. UCSF, San Francisco, USA

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1319)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
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  2. Yeast Surface Antibody Display Library and Antibody Engineering

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Protein Engineering and Selection Using Yeast Surface Display

      • Alessandro Angelini, Tiffany F. Chen, Seymour de Picciotto, Nicole J. Yang, Alice Tzeng, Michael S. Santos et al.
      Pages 3-36

    3. Isolation and Validation of Anti-B7-H4 scFvs from an Ovarian Cancer scFv Yeast-Display Library

      • Denarda Dangaj, Nathalie Scholler
      Pages 37-49

    4. Combining Phage and Yeast Cell Surface Antibody Display to Identify Novel Cell Type-Selective Internalizing Human Monoclonal Antibodies

      • Scott Bidlingmaier, Yang Su, Bin Liu
      Pages 51-63

    5. Yeast Display-Based Antibody Affinity Maturation Using Detergent-Solubilized Cell Lysates

      • Benjamin J. Tillotson, Jason M. Lajoie, Eric V. Shusta
      Pages 65-78

  3. Yeast Surface Display as a Tool for Protein Engineering

    1. Front Matter

      Pages 79-79
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    2. Yeast Endoplasmic Reticulum Sequestration Screening for the Engineering of Proteases from Libraries Expressed in Yeast

      • Li Yi, Joseph M. Taft, Qing Li, Mark C. Gebhard, George Georgiou, Brent L. Iverson
      Pages 81-93

    3. T Cell Receptor Engineering and Analysis Using the Yeast Display Platform

      • Sheena N. Smith, Daniel T. Harris, David M. Kranz
      Pages 95-141

    4. Epitope-Specific Binder Design by Yeast Surface Display

      • Jasdeep K. Mann, Sheldon Park
      Pages 143-154

    5. Applications of Yeast Surface Display for Protein Engineering

      • Gerald M. Cherf, Jennifer R. Cochran
      Pages 155-175

  5. Yeast Surface Display in Bioassay and Industrial Applications

    1. Front Matter

      Pages 215-215
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    2. Enzyme Evolution by Yeast Cell Surface Engineering

      • Natsuko Miura, Kouichi Kuroda, Mitsuyoshi Ueda
      Pages 217-232

    3. Electrochemical Glucose Biosensor Based on Glucose Oxidase Displayed on Yeast Surface

      • Hongwei Wang, Qiaolin Lang, Bo Liang, Aihua Liu
      Pages 233-243

    4. Coupling Binding to Catalysis: Using Yeast Cell Surface Display to Select Enzymatic Activities

      • Keya Zhang, Karan Bhuripanyo, Yiyang Wang, Jun Yin
      Pages 245-260

    5. The Use of Yeast Surface Display in Biofuel Cells

      • Alon Szczupak, Lital Alfonta
      Pages 261-268
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Keywords

About this book

In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering,  yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers. ​

Editors and Affiliations

  • UCSF, San Francisco, USA

    Bin Liu

Bibliographic Information

  • Book Title: Yeast Surface Display

  • Book Subtitle: Methods, Protocols, and Applications

  • Editors: Bin Liu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2748-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2747-0Published: 11 June 2015

  • Softcover ISBN: 978-1-4939-4667-9Published: 09 October 2016

  • eBook ISBN: 978-1-4939-2748-7Published: 09 June 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 271

  • Number of Illustrations: 13 b/w illustrations, 28 illustrations in colour

  • Topics: Microbiology

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