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Detection of Blotted Proteins

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Biji T. Kurien

    1. Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. R. Hal Scofield

    1. Department of Medicine, Oklahoma Medical Research Foundation, Oklahoma City, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1314)

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Table of contents (38 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Western Blotting: Origin and Ascent of the Species

    • W. Neal Burnette
    Pages 1-3

  3. Methods to Concentrate Proteins for Protein Isolation, Proteomic, and Peptidomic Evaluation

    • J. P. Dean Goldring
    Pages 5-18

  4. Measuring Protein Concentration on Nitrocellulose and After the Electrophoretic Transfer of Protein to Nitrocellulose

    • J. P. Dean Goldring
    Pages 19-25

  5. Detection of Blotted Proteins: Not All Blockers Are Created Equal

    • Vishal Kothari, Suresh T. Mathews
    Pages 27-32

  6. Protein Stains to Detect Antigen on Membranes

    • Anil Dsouza, R. Hal Scofield
    Pages 33-40

  7. Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting

    • F. Javier Alba, Salvador Bartolomé, Antonio Bermúdez, Joan-Ramon Daban
    Pages 41-50

  8. Rapid, Antibody-Free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation

    • Neil W. Charter, Joe Horecka, Chin-Yee Loh, Albert Doan, Tom Wehrman, Keith R. Olson
    Pages 51-61

  9. Use of Nonradioactive Detection Method for North- and South-Western Blot

    • Claudia Franke, Daniel Gräfe, Holger Bartsch, Michael P. Bachmann
    Pages 63-71

  10. Immunoblotting Using Radiolabeled Reagents for Detection

    • Holger Bartsch, Claudia Franke, Michael P. Bachmann
    Pages 73-78

  11. Immunoblotting of Antigens: Whole, Strip, and New-Line Nitrocellulose Membrane Immunoblotting Using the Chemiluminescence Technique

    • Yaser Dorri
    Pages 79-94

  12. Detection of Protein Carbonyls by Means of Biotin Hydrazide–Streptavidin Affinity Methods

    • Kenneth Hensley
    Pages 95-100

  13. Direct Immunodetection of Antigens Within the Precast Polyacrylamide Gel

    • Surbhi Desai, Boguslawa R. Dworecki, Marie C. Nlend
    Pages 101-114

  14. Quantitative Analysis of Signal Transduction with In-Cell Western Immunofluorescence Assays

    • Vince Boveia, Amy Schutz-Geschwender
    Pages 115-130

  15. Ultrasensitive Protein Detection on Dot Blots and Western Blots with Semiconducting Polymer Dots

    • Fangmao Ye, Polina B. Smith, Daniel T. Chiu
    Pages 131-137

  16. Co-detection of Target and Total Protein by CyDye Labeling and Fluorescent ECL Plex Immunoblotting in a Standard Proteomics Workflow

    • Caitriona Scaife, Ciara A. McManus, Pamela M. Donoghue, Michael J. Dunn
    Pages 139-149

  17. Using Biotinylated Proteins to Demonstrate Immunodetection of Antigens via Western Blotting, Dot Blots, and Immunohistochemistry

    • Thomas Millar, Ronald Knighton, Jo-Anne Chuck
    Pages 151-164

  18. Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane

    • Biji T. Kurien, Michael P. Bachmann
    Pages 165-171

  19. Sequential Use of Immunoblots for Characterization of Autoantibody Specificities

    • Holger Bartsch, Michael P. Bachmann
    Pages 173-178

  20. Nanogold Immunodetection Detection Systems for the Identification of Autoantigens by Western Blotting

    • Jacen S. Moore, R. Hal Scofield
    Pages 179-184
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Keywords

About this book

This detailed volume provides methods and techniques for detection after blotting.  Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Detection of Blotted Proteins: Methods and Protocols presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting.

Editors and Affiliations

  • Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, USA

    Biji T. Kurien

  • Department of Medicine, Oklahoma Medical Research Foundation, Oklahoma City, USA

    R. Hal Scofield

Bibliographic Information

  • Book Title: Detection of Blotted Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Biji T. Kurien, R. Hal Scofield

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2718-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2717-3Published: 04 July 2015

  • Softcover ISBN: 978-1-4939-5595-4Published: 15 October 2016

  • eBook ISBN: 978-1-4939-2718-0Published: 02 July 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 386

  • Number of Illustrations: 53 b/w illustrations, 48 illustrations in colour

  • Topics: Protein Science

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