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Plant Phosphoproteomics

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Waltraud X. Schulze

    1. Department of Plant Systems Biology, Universität Hohenheim, Stuttgart, Germany

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  • Includes cutting-edge methods and protocols in plant phosphoproteomics
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1306)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. The Plant Kinome

    • Monika Zulawski, Waltraud X. Schulze
    Pages 1-23

  3. Phosphatases in Plants

    • Alois Schweighofer, Irute Meskiene
    Pages 25-46

  4. Phosphoproteomics in Cereals

    • Pingfang Yang
    Pages 47-57

  5. Screening of Kinase Substrates Using Kinase Knockout Mutants

    • Taishi Umezawa
    Pages 59-69

  6. Phosphopeptide Profiling of Receptor Kinase Mutants

    • Xu Na Wu, Waltraud X. Schulze
    Pages 71-79

  7. Combining Metabolic 15N Labeling with Improved Tandem MOAC for Enhanced Probing of the Phosphoproteome

    • Martin Thomas, Nicola Huck, Wolfgang Hoehenwarter, Uwe Conrath, Gerold J. M. Beckers
    Pages 81-96

  8. Kinase Activity and Specificity Assay Using Synthetic Peptides

    • Xu Na Wu, Waltraud X. Schulze
    Pages 97-104

  9. Absolute Quantitation of Protein Posttranslational Modification Isoform

    • Zhu Yang, Ning Li
    Pages 105-119

  10. Phosphorylation Stoichiometry Determination in Plant Photosynthetic Membranes

    • Björn Ingelsson, Rikard Fristedt, Maria V. Turkina
    Pages 121-134

  11. Phosphopeptide Immuno-Affinity Enrichment to Enhance Detection of Tyrosine Phosphorylation in Plants

    • Sharon C. Mithoe, Frank L. H. Menke
    Pages 135-146

  12. The Peptide Microarray ChloroPhos1.0: A Screening Tool for the Identification of Arabidopsis thaliana Chloroplast Protein Kinase Substrates

    • Anna Schönberg, Sacha Baginsky
    Pages 147-157

  13. Plant Protein Kinase Substrates Identification Using Protein Microarrays

    • Shisong Ma, Savithramma P. Dinesh-Kumar
    Pages 159-165

  14. Targeted Analysis of Protein Phosphorylation by 2D Electrophoresis

    • Kristin Mayer, Sally Albrecht, Andreas Schaller
    Pages 167-176

  15. Computational Phosphorylation Network Reconstruction: Methods and Resources

    • Guangyou Duan, Dirk Walther
    Pages 177-194

  16. Computational Identification of Protein Kinases and Kinase-Specific Substrates in Plants

    • Han Cheng, Yongbo Wang, Zexian Liu, Yu Xue
    Pages 195-205

  17. Databases for Plant Phosphoproteomics

    • Waltraud X. Schulze, Qiuming Yao, Dong Xu
    Pages 207-216

  18. Phosphorylation Site Prediction in Plants

    • Qiuming Yao, Waltraud X. Schulze, Dong Xu
    Pages 217-228

  19. Back Matter

    Pages 229-231
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Keywords

About this book

This detailed volume addresses recent developments in phosphoproteomic techniques with a particular focus on the plant system. Over the recent decades, proteomic methods were refined to study the significance and dynamics of protein phosphorylation in various biological contexts. However, working with plant tissue imposes particular challenges to the biologist which are attributed to the rigid cell wall making protein extraction more difficult, the skewed protein abundance with Rubisco as a highly abundant protein and a large central vacuole leading to low protein yield and increased degradative enzyme activity. The methodologies in this book seek to move beyond these issues. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Plant Phosphoproteomics: Methods and Protocols serves as an ideal reference for researchers investigating this vital area of plant science.

Editors and Affiliations

  • Department of Plant Systems Biology, Universität Hohenheim, Stuttgart, Germany

    Waltraud X. Schulze

Bibliographic Information

  • Book Title: Plant Phosphoproteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Waltraud X. Schulze

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2648-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2647-3Published: 01 May 2015

  • Softcover ISBN: 978-1-4939-4937-3Published: 09 October 2016

  • eBook ISBN: 978-1-4939-2648-0Published: 30 April 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 231

  • Number of Illustrations: 8 b/w illustrations, 16 illustrations in colour

  • Topics: Plant Sciences, Proteomics

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