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Plant Mitochondria

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. James Whelan

    1. La Trobe University, Bundoora, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Monika W. Murcha

    1. The University of Western Australia, Crawley, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols in plant cell biology
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1305)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xxiii
    Download chapter PDF

  2. Isolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa

    • Monika W. Murcha, James Whelan
    Pages 1-12

  3. Determining Mitochondrial Transcript Termini for the Study of Transcription Start Sites and Transcript 5′ End Maturation

    • Stefan Binder, Kristina Kühn
    Pages 13-30

  4. Mitochondrial Run-On Transcription Assay Using Biotin Labeling

    • Kristina Kühn
    Pages 31-44

  5. In Vitro RNA Uptake Studies in Plant Mitochondria

    • Szymon Kubiszewski-Jakubiak, Cyrille Megel, Elodie Ubrig, Thalia Salinas, Anne-Marie Duchêne, Laurence Maréchal-Drouard
    Pages 45-60

  6. In Vitro and In Vivo Protein Uptake Studies in Plant Mitochondria

    • Owen Duncan, Chris Carrie, Yan Wang, Monika W. Murcha
    Pages 61-81

  7. Plant Mitochondrial Proteomics

    • Nicolas L. Taylor, A. Harvey Millar
    Pages 83-106

  8. Identification of Lysine-Acetylated Mitochondrial Proteins and Their Acetylation Sites

    • Markus Hartl, Ann-Christine König, Iris Finkemeier
    Pages 107-121

  9. A Flowchart to Analyze Protease Activity in Plant Mitochondria

    • Pedro F. Teixeira, Rui M. Branca, Beata Kmiec, Elzbieta Glaser
    Pages 123-130

  10. Activity Measurements of Mitochondrial Enzymes in Native Gels

    • Peter Schertl, Hans-Peter Braun
    Pages 131-138

  11. Activity Assay for Plant Mitochondrial Enzymes

    • Shaobai Huang, Chun Pong Lee, A. Harvey Millar
    Pages 139-149

  12. Analysis of Type II NAD(P)H Dehydrogenases

    • Kathleen L. Soole, Chevaun A. Smith
    Pages 151-164

  13. Assessment of Respiration in Isolated Plant Mitochondria Using Clark-Type Electrodes

    • Richard P. Jacoby, A. Harvey Millar, Nicolas L. Taylor
    Pages 165-185

  14. Micro-Respiratory Measurements in Plants

    • Yun Shin Sew, A. Harvey Millar, Elke Stroeher
    Pages 187-196

  15. Improvements to Define Mitochondrial Metabolomics Using Nonaqueous Fractionation

    • Richard Fly, James Lloyd, Stephan Krueger, Alisdair Fernie, Margaretha J. van der Merwe
    Pages 197-210

  16. Mitochondrial Markers of Programmed Cell Death in Arabidopsis thaliana

    • Theresa J. Reape, Joanna Kacprzyk, Niall Brogan, Lee Sweetlove, Paul F. McCabe
    Pages 211-221

  17. Imaging and Analysis of Mitochondrial Dynamics in Living Cells

    • Sanjaya B. Ekanayake, Amr M. El Zawily, Gaël Paszkiewicz, Aurélia Rolland, David C. Logan
    Pages 223-240

  18. Analysis of Plant Mitochondrial Function Using Fluorescent Protein Sensors

    • Stephan Wagner, Thomas Nietzel, Isabel Aller, Alex Costa, Mark D. Fricker, Andreas J. Meyer et al.
    Pages 241-252

  19. In Planta Analysis of Leaf Mitochondrial Superoxide and Nitric Oxide

    • Marina Cvetkovska, Greg C. Vanlerberghe
    Pages 253-261

  20. Databases and Informatics Resources for Analysis of Plant Mitochondria

    • Reena Narsai
    Pages 263-279
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Keywords

About this book

The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein and enzymatic activities. Given that the ability to uncover mitochondria’s unique features is underpinned by current methodology, this book explores the subject from morphology to detailed molecular mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Plant Mitochondria: Methods and Protocols serves as a vital resource to beginners in the field as well as to expert researchers who find themselves being pulled into the field of mitochondrial research as it links to so many important aspects of plant cell biology.

Editors and Affiliations

  • La Trobe University, Bundoora, Australia

    James Whelan

  • The University of Western Australia, Crawley, Australia

    Monika W. Murcha

Bibliographic Information

  • Book Title: Plant Mitochondria

  • Book Subtitle: Methods and Protocols

  • Editors: James Whelan, Monika W. Murcha

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2639-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2638-1Published: 25 April 2015

  • Softcover ISBN: 978-1-4939-4906-9Published: 26 June 2017

  • eBook ISBN: 978-1-4939-2639-8Published: 24 April 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXIII, 303

  • Number of Illustrations: 12 b/w illustrations, 28 illustrations in colour

  • Topics: Plant Sciences

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