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Multiple Sclerosis

Methods and Protocols

Editors:

  • Features techniques for researching multiple sclerosis on a molecular and cellular level
  • Includes step-by-step methodologies that have been tested in labs around the world
  • Provides key tips and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1304)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
  2. Positional Gene Cloning in Experimental Populations

    • Maja Jagodic, Pernilla Stridh
    Pages 3-24
  3. Generation of Transgenic Rats Using Lentiviral Vectors

    • Holger M. Reichardt, Henrike J. Fischer
    Pages 25-37
  4. DNA Vaccination Techniques

    • Nicolás Fissolo, Xavier Montalban, Manuel Comabella
    Pages 39-50
  5. Peripheral Blood Mononuclear Cells: Isolation, Freezing, Thawing, and Culture

    • Christine Riedhammer, Dagmar Halbritter, Robert Weissert
    Pages 53-61
  6. ELISPOT Techniques

    • Niannian Ji, Thomas G. Forsthuber
    Pages 63-71
  7. Isolation of Central Nervous System (CNS) Infiltrating Cells

    • Ilgiz A. Mufazalov, Ari Waisman
    Pages 73-79
  8. Methods for Ex Vivo Analysis of Immune Cell Function from the Central Nervous System

    • Darryl G. Turner, Melanie D. Leech, Richard A. O’Connor, Stephen M. Anderton
    Pages 81-90
  9. Neurofilament Light Chain Determination from Peripheral Blood Samples

    • Marguerite Limberg, Giulio Disanto, Christian Barro, Jens Kuhle
    Pages 93-98
  10. In Vivo Visualization of (Auto)Immune Processes in the Central Nervous System of Rodents

    • Christian Schläger, Tanja Litke, Alexander Flügel, Francesca Odoardi
    Pages 117-129
  11. Experimental Autoimmune Encephalomyelitis in Mice

    • Rachael L. Terry, Igal Ifergan, Stephen D. Miller
    Pages 145-160
  12. Experimental Autoimmune Encephalomyelitis in Marmosets

    • S. Anwar Jagessar, Karin Dijkman, Jordon Dunham, Bert A. ‘t Hart, Yolanda S. Kap
    Pages 171-186
  13. Back Matter

    Pages 231-232

About this book

This detailed volume covers molecular biology, cellular biology, biomarkers, imaging, and neuropathological methods and techniques to explore multiple sclerosis (MS), with a special emphasis on disease models. With so much effort needed to elucidate basic disease mechanisms, to clone disease relevant genes, to define novel biomarkers, and to discover novel and improved therapeutic and curative treatments, this book serves to aid researchers in accomplishing these enormous goals.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and easy to use, Multiple Sclerosis: Methods and Protocols will empower the reader to perform novel research regarding pathophysiology and treatment for MS.

Editors and Affiliations

  • University of Regensburg, Regensburg, Germany

    Robert Weissert

About the editor

Prof. Robert Weissert, M.D. Ph.D. University Professor of Clinical Neurobiology Department of Neurology University of Regensburg Universitätsstraße 84 93053 Regensburg Germany Phone: +49-941-9418721 E-mail: robert.weissert@klinik.uni-regensburg.de

Bibliographic Information

  • Book Title: Multiple Sclerosis

  • Book Subtitle: Methods and Protocols

  • Editors: Robert Weissert

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2630-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-2629-9Published: 07 August 2015

  • Softcover ISBN: 978-1-4939-4938-0Published: 19 October 2016

  • eBook ISBN: 978-1-4939-2630-5Published: 06 August 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 232

  • Number of Illustrations: 19 b/w illustrations, 18 illustrations in colour

  • Topics: Molecular Medicine, Neurology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access