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  • Book
  • © 2015

Plant Pathology

Techniques and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1302)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xi
  2. Detection of the Bacterial Potato Pathogens Pectobacterium and Dickeya spp. Using Conventional and Real-Time PCR

    • Sonia N. Humphris, Greig Cahill, John G. Elphinstone, Rachel Kelly, Neil M. Parkinson, Leighton Pritchard et al.
    Pages 1-16
  3. Diagnosis of Ramularia collo-cygni and Rhynchosporium spp. in Barley

    • Neil Havis, James Fountaine, Kalina Gorniak, Linda Paterson, Jeanette Taylor
    Pages 29-36
  4. Diagnostics of Tree Diseases Caused by Phytophthora austrocedri Species

    • Vincent Mulholland, Matthew Elliot, Sarah Green
    Pages 59-74
  5. Real-Time LAMP for Chalara fraxinea Diagnosis

    • Jenny Tomlinson, Neil Boonham
    Pages 75-83
  6. Diagnosis of Phytoplasmas by Real-Time PCR Using Locked Nucleic Acid (LNA) Probes

    • Sabrina Palmano, Vincent Mulholland, David Kenyon, Gerry S. Saddler, Colin Jeffries
    Pages 113-122
  7. Q-Bank Phytoplasma: A DNA Barcoding Tool for Phytoplasma Identification

    • Nicoletta Contaldo, Samanta Paltrinieri, Olga Makarova, Assunta Bertaccini, Mogens Nicolaisen
    Pages 123-135
  8. High-Throughput Diagnosis of Potato Cyst Nematodes in Soil Samples

    • Alex Reid, Fiona Evans, Vincent Mulholland, Yvonne Cole, Jon Pickup
    Pages 137-148
  9. Detection of Nepovirus Vector and Nonvector Xiphinema Species in Grapevine

    • C. Van Ghelder, A. Reid, D. Kenyon, D. Esmenjaud
    Pages 149-159
  10. Molecular and Serological Methods for the Diagnosis of Viruses in Potato Tubers

    • Christophe Lacomme, Ross Holmes, Fiona Evans
    Pages 161-176
  11. SNaPshot and CE-SSCP: Two Simple and Cost-Effective Methods to Reveal Genetic Variability Within a Virus Species

    • Agnès Delaunay, Sylvie Dallot, Denis Filloux, Virginie Dupuy, Philippe Roumagnac, Emmanuel Jacquot
    Pages 187-206
  12. Virus Testing by PCR and RT-PCR Amplification in Berry Fruit

    • Stuart MacFarlane, Wendy McGavin, Ioannis Tzanetakis
    Pages 227-248
  13. Metagenomics Approaches Based on Virion-Associated Nucleic Acids (VANA): An Innovative Tool for Assessing Without A Priori Viral Diversity of Plants

    • Denis Filloux, Sylvie Dallot, Agnès Delaunay, Serge Galzi, Emmanuel Jacquot, Philippe Roumagnac
    Pages 249-257

About this book

The second edition of Plant Pathology: Techniques and Protocols covers diagnostic methods that are currently used in laboratories for a broad range of plant species and matrixes. These include serological and molecular methods that have one or more of the following characteristics: suitability for high-throughput testing, detection of a group of pathogens or of sometimes uncharacterized pathogens, detection and identification of specific pathogens, and high sensitivity. This volume discusses qualitative and quantitative tests, as well as recently developed diagnostic methods. It also provides background information on many pathogens, which are either endemic, non-endemic, or emerging and with different lifecycles that cause diseases of significant importance in a wide variety of hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, background information on pathogens and the disease caused, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Informative and cutting-edge, Plant Pathology: Techniques and Protocols, Second Edition is the perfect book for plant pathologists and molecular biologists who will use this information to test out the latest research in their laboratories. 

Editors and Affiliations

  • Virology & Zoology, SASA, Edinburgh, United Kingdom

    Christophe Lacomme

Bibliographic Information

  • Book Title: Plant Pathology

  • Book Subtitle: Techniques and Protocols

  • Editors: Christophe Lacomme

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2620-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2619-0Published: 19 May 2015

  • Softcover ISBN: 978-1-4939-4925-0Published: 29 October 2016

  • eBook ISBN: 978-1-4939-2620-6Published: 15 May 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 352

  • Number of Illustrations: 20 b/w illustrations, 47 illustrations in colour

  • Topics: Plant Pathology, Plant Sciences

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access