Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1298)
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Table of contents (29 protocols)
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Front Matter
Keywords
About this book
This volume covers the latest technological advances in the characterization of the biosynthesis and functions of Rab GTPases and their regulation by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The book consists of 28 chapters and starts with an overview of the Rab GTPase family. The next few chapters describe systematic approaches to the identification and classification of Rabs and Rab GAPs, as well as the detection of Rab isoprenylation and membrane distribution. The last few chapters examine the biochemical and functional properties of individual Rabs in the order of exocytic, recycling and endocytic Rabs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.
Instructive and practical, Rab GTPases: Methods and Protocols approaches each topic with great detail and is a valuable resource for researchers and students interested in the field of Rab GTPases.
Editors and Affiliations
About the editor
Bibliographic Information
Book Title: Rab GTPases
Book Subtitle: Methods and Protocols
Editors: Guangpu Li
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2569-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2568-1Published: 25 March 2015
Softcover ISBN: 978-1-4939-5594-7Published: 05 October 2016
eBook ISBN: 978-1-4939-2569-8Published: 23 March 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 358
Number of Illustrations: 28 b/w illustrations, 54 illustrations in colour
Topics: Biochemistry, general, Membrane Biology