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RNA Nanotechnology and Therapeutics

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Peixuan Guo

    1. Nanobiotechnology Center, Markey Cancer Center, Department of Pharmaceutical Sciences, University of Kentucky, Lexington, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Farzin Haque

    1. Nanobiotechnology Center, Markey Cancer Center, Department of Pharmaceutical Sciences, University of Kentucky, Lexington, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1297)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Overview of Methods in RNA Nanotechnology: Synthesis, Purification, and Characterization of RNA Nanoparticles

    • Farzin Haque, Peixuan Guo
    Pages 1-19

  3. Multiple Approaches for the Investigation of Bacterial Small Regulatory RNAs Self-assembly

    • Christophe Lavelle, Florent Busi, Véronique Arluison
    Pages 21-42

  4. Measuring the Elasticity of Ribonucleotide(s)-Containing DNA Molecules Using AFM

    • Kyung Duk Koh, Hsiang-Chih Chiu, Elisa Riedo, Francesca Storici
    Pages 43-57

  5. Silver Nanoclusters for RNA Nanotechnology: Steps Towards Visualization and Tracking of RNA Nanoparticle Assemblies

    • Kirill A. Afonin, Danielle Schultz, Luc Jaeger, Elisabeth Gwinn, Bruce A. Shapiro
    Pages 59-66

  6. Large Scale Purification of RNA Nanoparticles by Preparative Ultracentrifugation

    • Daniel L. Jasinski, Chad T. Schwartz, Farzin Haque, Peixuan Guo
    Pages 67-82

  7. HPLC Purification of RNA Aptamers up to 59 Nucleotides with Single-Nucleotide Resolution

    • Zhen Huang, Chi-Yen Lin, William Jaremko, Li Niu
    Pages 83-93

  8. Using RNA Nanoparticles with Thermostable Motifs and Fluorogenic Modules for Real-Time Detection of RNA Folding and Turnover In Prokaryotic and Eukaryotic Cells

    • Hui Zhang, Fengmei Pi, Dan Shu, Mario Vieweger, Peixuan Guo
    Pages 95-111

  9. Fluorescence Labeling of Short RNA by Oxidation at the 3′-End

    • Chen Qiu, Wang-Yi Liu, Yong-Zhen Xu
    Pages 113-120

  10. Methods and Assays for Specific Targeting and Delivery of RNA Nanoparticles to Cancer Metastases

    • Piotr Rychahou, Yi Shu, Farzin Haque, Jiyao Hu, Peixuan Guo, B. Mark Evers
    Pages 121-135

  11. Functional Assays for Specific Targeting and Delivery of RNA Nanoparticles to Brain Tumor

    • Tae Jin Lee, Farzin Haque, Mario Vieweger, Ji Young Yoo, Balveen Kaur, Peixuan Guo et al.
    Pages 137-152

  12. Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide–Protein Complexes to SELEX Screens

    • Laetitia Evadé, Eric Dausse, Said Taouji, Emilie Daguerre, Eric Chevet, Jean-Jacques Toulmé
    Pages 153-167

  13. Methods for Assembling B-Cell Lymphoma Specific and Internalizing Aptamer–siRNA Nanoparticles Via the Sticky Bridge

    • Jiehua Zhou, John J. Rossi, Ka To Shum
    Pages 169-185

  14. A High-Throughput Screening Assay for the Functional Delivery of Splice-Switching Oligonucleotides in Human Melanoma Cells

    • John M. Dean, Robert K. DeLong
    Pages 187-196

  15. Design, Assembly, and Evaluation of RNA–Protein Nanostructures

    • Hirohisa Ohno, Eriko Osada, Hirohide Saito
    Pages 197-211

  16. Mapping RNA Interactions to Proteins in Virions Using CLIP-Seq

    • Baochang Fan, Peng Ni, C. Cheng Kao
    Pages 213-224

  17. Mapping Protein–RNA Interactions by RCAP, RNA-Cross-Linking and Peptide Fingerprinting

    • Robert C. Vaughan, C. Cheng Kao
    Pages 225-236

  18. Back Matter

    Pages 237-239
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Keywords

About this book

This volume contains a compilation of techniques and laboratory protocols directly related to RNA nano technology and its applications in nano biotechnology and nano medicine. The chapters in this book cover a wide range of research methods that can be easily comprehended and carried out in a step-by-step manner by graduate students and postdoctoral fellows from diverse scientific disciplines. Some of the covered topics include various biochemical, biophysical and bioinformatics approaches for analyzing RNA structures and properties; methods to analyze the multi-step assembly process of RNA nano architectures; purification of multifunctional RNA nanoparticles by ultracentrifugation and HPLC; real-time detection of RNA nano constructs in vivo; conjugation of imaging, targeting and therapeutic modules to RNA scaffold; and design and characterization of RNA-protein nanostructures. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubles troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, RNA Nanotechnology and Therapeutics: Methods and Protocols propels the field toward new heights by engaging researchers working in the diverse fields of physical, engineering, and life sciences.

Editors and Affiliations

  • Nanobiotechnology Center, Markey Cancer Center, Department of Pharmaceutical Sciences, University of Kentucky, Lexington, USA

    Peixuan Guo, Farzin Haque

About the editors

Peixuan Guo, Ph. D. William Farish Endowed Chair in Nano biotechnology Professor of School of Pharmacy Director of Nano biotechnology Center University of Kentucky Director of NCI Cancer Nanotechnology Platform Partnership Program: RNA Nanotechnology for Cancer Therapy Mailing address College of Pharmacy University of Kentucky 565 Biopharm Complex 789 S. Limestone Street Lexington, KY 40536 Phone: (Office) (859)218-0128 (Cell Phone): (513)728-1411 peixuan.guo@uky.edu (future)

Bibliographic Information

  • Book Title: RNA Nanotechnology and Therapeutics

  • Book Subtitle: Methods and Protocols

  • Editors: Peixuan Guo, Farzin Haque

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2562-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2561-2Published: 21 April 2015

  • Softcover ISBN: 978-1-4939-5450-6Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2562-9Published: 20 April 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 239

  • Number of Illustrations: 20 b/w illustrations, 49 illustrations in colour

  • Topics: Biotechnology, Nanotechnology

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