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Small Non-Coding RNAs

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Mathieu Rederstorff

    1. Université de Lorraine, Vandoeuvre-lès-Nancy, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1296)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Introduction to Small Non-Coding RNAs

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Small Non-Coding RNAs: A Quick Look in the Rearview Mirror

      • Guillaume Clerget, Yoann Abel, Mathieu Rederstorff
      Pages 3-9

    3. Alcoholic Precipitation of Small Non-Coding RNAs

      • Guillaume Clerget, Valérie Bourguignon-Igel, Mathieu Rederstorff
      Pages 11-16

    4. Quantification and Quality Control of a Small Non-Coding RNA Preparation

      • Virginie Marchand, Christiane Branlant
      Pages 17-28

    5. Impact of RNA Isolation Protocols on RNA Detection by Northern Blotting

      • Katrin Damm, Simone Bach, Katrin M. H. Müller, Gabriele Klug, Olga Y. Burenina, Elena A. Kubareva et al.
      Pages 29-38

  3. Visualization and Analysis of Small Non-coding RNAs

    1. Front Matter

      Pages 39-39
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    2. Improved Northern Blot Detection of Small RNAs Using EDC Crosslinking and DNA/LNA Probes

      • Katrin Damm, Simone Bach, Katrin M. H. Müller, Gabriele Klug, Olga Y. Burenina, Elena A. Kubareva et al.
      Pages 41-51

    3. Direct Cloning of Double-Stranded RNAs

      • Manli Shen, Marina Falaleeva, Natalia Korotkova, Stefan Stamm
      Pages 53-64

    4. Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation

      • Gabrielle Bourgeois, Florian Chardon, Anne-Sophie Tillault, Magali Blaud
      Pages 65-72

    5. Fluorescence In Situ Hybridization of Small Non-Coding RNAs

      • Valentin Vautrot, Christelle Aigueperse, Christiane Branlant, Isabelle Behm-Ansmant
      Pages 73-83

    6. RT-qPCR-Based Quantification of Small Non-Coding RNAs

      • Fjoralba Zeka, Pieter Mestdagh, Jo Vandesompele
      Pages 85-102

    7. Stem-Loop RT-PCR Based Quantification of Small Non-Coding RNAs

      • Véronique Salone, Mathieu Rederstorff
      Pages 103-108

    8. miR-RACE: An Effective Approach to Accurately Determine the Sequence of Computationally Identified miRNAs

      • Chen Wang, Jinggui Fang
      Pages 109-118

    9. Probing Small Non-Coding RNAs Structures

      • Jean-Vincent Philippe, Lilia Ayadi, Christiane Branlant, Isabelle Behm-Ansmant
      Pages 119-136

  4. High-Throughput Approaches to Study Non-coding RNAs

    1. Front Matter

      Pages 137-137
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    2. cDNA Library Generation for the Analysis of Small RNAs by High-Throughput Sequencing

      • Jennifer Gebetsberger, Roger Fricker, Norbert Polacek
      Pages 139-149

    3. CLIP-Seq to Discover Transcriptome-Wide Imprinting of RNA Binding Proteins in Living Cells

      • Jérôme Saulière, Hervé Le Hir
      Pages 151-160

    4. Microarray Analysis of Small Non-Coding RNAs

      • Michael Karbiener, Marcel Scheideler
      Pages 161-171

  5. Small Non-coding RNAs Applications

    1. Front Matter

      Pages 173-173
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Keywords

About this book

This volume contains state-of-the-art methods tackling all aspects of small non-coding RNAs biology.  Small Non-Coding RNAs: Methods and Protocols guides readers through customized dedicated protocols and technologies that will be of valuable help to all those willing to contribute deciphering the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubles troubleshooting and avoiding known pitfalls.

Instructive and practical, Small Non-Coding RNAs: Methods and Protocols reaches out to biochemists, cellular and molecular biologists already working in the field of RNA biology  and to those just starting to study small non-coding RNAs.

Editors and Affiliations

  • Université de Lorraine, Vandoeuvre-lès-Nancy, France

    Mathieu Rederstorff

About the editor

Mathieu Rederstorff, PhD Associate Professor Laboratoire IMoPA UMR 7365 CNRS/UL Equipe ARN, RNP - Maturation, Structure, Fonction - Université de Lorraine Biopôle/Campus Médecine Brabois 9 Avenue de la Forêt de Haye BP 184 54506 Vandoeuvre-lès-Nancy FRANCE Tel: +33 3 83 68 55 31 (office) +33 3 83 68 55 25 (lab) +33 6 49 12 67 11 (mobile) Fax: +33 3 83 68 55 09 (fax) mathieu.rederstorff@univ-lorraine.fr mathieu.rederstorff@maem.uhp-nancy.fr

Bibliographic Information

  • Book Title: Small Non-Coding RNAs

  • Book Subtitle: Methods and Protocols

  • Editors: Mathieu Rederstorff

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2547-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2546-9Published: 21 March 2015

  • Softcover ISBN: 978-1-4939-4903-8Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2547-6Published: 19 March 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 238

  • Number of Illustrations: 20 b/w illustrations, 15 illustrations in colour

  • Topics: Human Genetics

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