Overview
- Includes cutting-edge methods and protocols on cAMP signaling
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1294)
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Table of contents (16 protocols)
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Front Matter
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Back Matter
Keywords
About this book
This detailed volume encompasses new technological developments that specifically address questions related to adenosine 3′,5′-monophosphate (cAMP) compartmentalization, that probe relevant protein-protein interactions, that increase the spatial and temporal resolution of cAMP signal detection, and that can facilitate integration of the mounting complexity of the information that is becoming available on this signaling system. cAMP, the prototypical intracellular second messenger, regulates a large variety of cellular functions and biological processes, including gene transcription, cell metabolism, proliferation, development, as well as more specialized functions depending on the cell type, so the realization of its extremely complex spatial organization and local regulation is providing novel mechanistic insight into cell physiology and is producing a novel framework for the identification of disease mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, cAMP Signaling: Methods and Protocols serves as a vital resource for researchers working in this expanding, complex field.
Editors and Affiliations
Bibliographic Information
Book Title: cAMP Signaling
Book Subtitle: Methods and Protocols
Editors: Manuela Zaccolo
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2537-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2536-0Published: 19 March 2015
Softcover ISBN: 978-1-4939-4950-2Published: 05 October 2016
eBook ISBN: 978-1-4939-2537-7Published: 17 March 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 222
Number of Illustrations: 11 b/w illustrations, 36 illustrations in colour
Topics: Cell Biology