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T follicular Helper Cells

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Marion Espéli

    1. INSERM UMR_S996, LabEx LERMIT, Université Paris-Sud, Clamart, France

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    You can also search for this editor in PubMed   Google Scholar

  2. Michelle Linterman

    1. Lymphocyte Signalling and Development ISP Babraham Institute, Babraham Research Campus, Cambridge, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1291)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Mouse

    1. Front Matter

      Pages 1-1
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    2. Identification of Mouse T Follicular Helper Cells by Flow Cytometry

      • Alexandre P. Meli, Irah L. King
      Pages 3-11

    3. Identification of Follicular T Helper Cells in Tissue Sections

      • Yanna Ding, John D. Mountz, Hui-Chen Hsu
      Pages 13-25

    4. Tracking Early T Follicular Helper Cell Differentiation In Vivo

      • Dirk Baumjohann, K. Mark Ansel
      Pages 27-38

    5. Tracking by Flow Cytometry Antigen-Specific Follicular Helper T Cells in Wild-Type Animals After Protein Vaccination

      • Svetoslav Chakarov, Nicolas Fazilleau
      Pages 39-47

    6. Retroviral Vector Expression in TCR Transgenic CD4+ T Cells

      • Youn Soo Choi, Shane Crotty
      Pages 49-61

    7. Two-Photon Microscopy for Imaging Germinal Centers and T Follicular Helper Cells

      • Menna R. Clatworthy
      Pages 63-75

    8. In Vivo Induction of T-Follicular Helper Cells by Modulation of Regulatory T Cell Function

      • James Badger Wing, Shimon Sakaguchi
      Pages 77-85

    9. Assessing T Follicular Helper Cell Function In Vivo: Antigen-Specific B Cell Response to Hapten and Affinity Maturation

      • Jessica Natt, Marion Espéli
      Pages 87-101

    10. The SWHEL System for High-Resolution Analysis of In Vivo Antigen-Specific T-Dependent B Cell Responses

      • R. Brink, D. Paus, K. Bourne, J. R. Hermes, S. Gardam, T. G. Phan et al.
      Pages 103-123

    11. Triggering Positive Selection of Germinal Center B Cells by Antigen Targeting to DEC-205

      • Giulia Pasqual, Alessandro Angelini, Gabriel D. Victora
      Pages 125-134

    12. Detection of Mouse Natural Killer T Follicular Helper (NKTFH) Cells by Flow Cytometry

      • Pheh-Ping Chang, Carola G. Vinuesa
      Pages 135-141

    13. Identification of Foxp3+ T Follicular Regulatory (Tfr) Cells by Flow Cytometry

      • Ana Raquel Maceiras, Luis Graca
      Pages 143-150

    14. In Vitro Assay to Sensitively Measure Tfr Suppressive Capacity and Tfh Stimulation of B Cell Responses

      • Peter T. Sage, Arlene H. Sharpe
      Pages 151-160

  3. Human

    1. Front Matter

      Pages 161-161
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    2. Flow Cytometric Detection and Isolation of Human Tonsil or Lymph Node T Follicular Helper Cells

      • Jan Misiak, Karin Tarte, Patricia Amé-Thomas
      Pages 163-173

    3. Human Tfh and Tfr Cells: Identification and Assessment of Their Migration Potential

      • Chang H. Kim, Seika Hashimoto-Hill, Seung G. Kang
      Pages 175-186

    4. Analysis of Human Blood Memory T Follicular Helper Subsets

      • Salah-Eddine Bentebibel, Clement Jacquemin, Nathalie Schmitt, Hideki Ueno
      Pages 187-197

    5. Flow Cytometric Analysis of Circulating Follicular Helper T (Tfh) and Follicular Regulatory T (Tfr) Populations in Human Blood

      • Yunbo Wei, Jinhong Feng, Zhaohua Hou, Xin Maggie Wang, Di Yu
      Pages 199-207
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Keywords

About this book

This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward.

Editors and Affiliations

  • INSERM UMR_S996, LabEx LERMIT, Université Paris-Sud, Clamart, France

    Marion Espéli

  • Lymphocyte Signalling and Development ISP Babraham Institute, Babraham Research Campus, Cambridge, United Kingdom

    Michelle Linterman

Bibliographic Information

  • Book Title: T follicular Helper Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Marion Espéli, Michelle Linterman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2498-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2497-4Published: 03 April 2015

  • Softcover ISBN: 978-1-4939-5593-0Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2498-1Published: 02 April 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 228

  • Number of Illustrations: 25 b/w illustrations, 26 illustrations in colour

  • Topics: Cell Physiology

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