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Salamanders in Regeneration Research

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Anoop Kumar

    1. Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  2. András Simon

    1. Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1290)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-x
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  2. Salamanders

    1. Front Matter

      Pages 1-1
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    2. Variation in Salamanders: An Essay on Genomes, Development, and Evolution

      • Jeremy P. Brockes
      Pages 3-15

    3. Maintaining Eastern Newts (Notophthalmus viridescens) for Regeneration Research

      • Hans-Georg Simon, Shannon Odelberg
      Pages 17-25

    4. Housing and Maintenance of Ambystoma mexicanum, the Mexican Axolotl

      • Johanna E. Farkas, James R. Monaghan
      Pages 27-46

    5. Husbandry of Spanish Ribbed Newts (Pleurodeles waltl)

      • Alberto Joven, Matthew Kirkham, András Simon
      Pages 47-70

    6. Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies

      • Claudia Marcela Arenas, Andrea Gómez-Molina, Jean Paul Delgado
      Pages 71-78

  3. Experimental Manipulation in Salamanders

    1. Front Matter

      Pages 79-79
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    2. Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISH

      • Nobuyasu Maki
      Pages 81-89

    3. Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablation

      • Matthew Kirkham, Alberto Joven
      Pages 91-99

    4. The Accessory Limb Model: An Alternative Experimental System of Limb Regeneration

      • Tetsuya Endo, David M. Gardiner, Aki Makanae, Akira Satoh
      Pages 101-113

    5. High-Efficiency Electroporation of the Spinal Cord in Larval Axolotl

      • Aida Rodrigo Albors, Elly M. Tanaka
      Pages 115-125

    6. Pseudotyped Retroviruses for Infecting Axolotl

      • Tzu-Hsing Kuo, Jessica L. Whited
      Pages 127-140

    7. Thyroxine-Induced Metamorphosis in the Axolotl (Ambystoma mexicanum)

      • Peggy S. Coots, Ashley W. Seifert
      Pages 141-145

    8. Generation of Aneurogenic Larvae by Parabiosis of Salamander Embryos

      • Anoop Kumar, Jean Paul Delgado
      Pages 147-157

    9. In Vivo Modulation and Quantification of microRNAs During Axolotl Tail Regeneration

      • Jami R. Erickson, Karen Echeverri
      Pages 159-167

  4. Salamander Cells in Culture

    1. Front Matter

      Pages 169-169
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    2. Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemas

      • Patrizia Ferretti, Anoop Kumar
      Pages 171-185

    3. Culture and Transfection of Axolotl Cells

      • Jean-François Denis, Fadi Sader, Patrizia Ferretti, Stéphane Roy
      Pages 187-196

    4. Isolation and Culture of Neurospheres from the Adult Newt Brain

      • Liyakath Ali Shahul Hameed, András Simon
      Pages 197-204
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Keywords

About this book

This detailed volume focuses on best practices and conditions for maintaining the most commonly used salamander species in the laboratory. Salamanders in Regeneration Research: Methods and Protocols guides readers through experimental manipulations in vivo and in vitro, respectively. With methods on targeting a wide variety of structures, ranging from the limb to the heart and to the brain, and methods for studying genetically modified organisms and tools for mining in the genomic databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Salamanders in Regeneration Research: Methods and Protocols provides a comprehensive collection of methods chapters.

Editors and Affiliations

  • Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London, United Kingdom

    Anoop Kumar

  • Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden

    András Simon

Bibliographic Information

  • Book Title: Salamanders in Regeneration Research

  • Book Subtitle: Methods and Protocols

  • Editors: Anoop Kumar, András Simon

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2495-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2494-3Published: 05 March 2015

  • Softcover ISBN: 978-1-4939-4947-2Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2495-0Published: 04 March 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 357

  • Number of Illustrations: 8 b/w illustrations, 81 illustrations in colour

  • Topics: Cell Biology

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