Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1287)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (22 protocols)
-
Front Matter
-
Review Chapters
-
Front Matter
-
-
Methods Chapter
-
Front Matter
-
About this book
This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.
Editors and Affiliations
-
Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, USA
Kirankumar S. Mysore
-
National Institute of Plant Genome Research, New Delhi, India
Muthappa Senthil-Kumar
Bibliographic Information
Book Title: Plant Gene Silencing
Book Subtitle: Methods and Protocols
Editors: Kirankumar S. Mysore, Muthappa Senthil-Kumar
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2453-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2452-3Published: 05 March 2015
Softcover ISBN: 978-1-4939-4847-5Published: 05 October 2016
eBook ISBN: 978-1-4939-2453-0Published: 04 March 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 307
Number of Illustrations: 17 b/w illustrations, 17 illustrations in colour
Topics: Plant Genetics and Genomics