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  • © 2015

NF-kappa B

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1280)

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Table of contents (38 protocols)

  1. Front Matter

    Pages i-xvi
  2. Standard Approaches to Detect NF-κB Pathway Activation

    1. Front Matter

      Pages 1-1
    2. Electrophoretic Mobility Shift Assay Analysis of NF-κB DNA Binding

      • Sitharam Ramaswami, Matthew S. Hayden
      Pages 3-13
    3. Detection of IκB Degradation Dynamics and IκB-α Ubiquitination

      • Petro Starokadomskyy, Ezra Burstein
      Pages 15-24
  3. Detection and Analysis of NF-κB Signaling

    1. Front Matter

      Pages 45-45
    2. Assessing Sites of NF-κB DNA Binding Using Chromatin Immunoprecipitation

      • Amy Colleran, Patricia E. Collins, Ruaidhrí J. Carmody
      Pages 47-59
    3. IKK Kinase Assay for Assessment of Canonical NF-κB Activation in Neurons

      • Anca B. Mihalas, Mollie K. Meffert
      Pages 61-74
    4. Analysis of Epidermal Growth Factor-Induced NF-κB Signaling

      • Changying Jiang, Xin Lin
      Pages 75-102
    5. Systematic Detection of Noncanonical NF-κB Activation

      • Zhaoxia Qu, Gutian Xiao
      Pages 121-154
    6. Stable Reconstitution of IKK-Deficient Mouse Embryonic Fibroblasts

      • Carolyn M. Gray, Michael J. May
      Pages 181-195
  4. Methods to Study the Control of NF-κB Signaling

    1. Front Matter

      Pages 217-217
    2. Detection of Recombinant and Cellular MALT1 Paracaspase Activity

      • Daniel Nagel, Daniel Krappmann
      Pages 239-246
    3. TRAF Protein Function in Noncanonical NF-κB Signaling

      • Anna D. Reichardt, Jose Pindado, Shivam A. Zaver, Genhong Cheng
      Pages 247-268
    4. Roles of c-IAP Proteins in TNF Receptor Family Activation of NF-κB Signaling

      • Eugene Varfolomeev, Tatiana Goncharov, Domagoj Vucic
      Pages 269-282

About this book

Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-κB activation, regulation, and function. NF-kappa B: Methods and Protocols guides readers through standard approaches to detect NF-κB pathway activation, detection and analysis of NF-κB signaling, and methods to study the control of NF-κB signaling. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, NF-Kappa B: Methods and Protocols provides a timely and invaluable resource for researchers seeking to perform experiments aimed at understanding the role of NF-κB signaling in health and disease.

Editors and Affiliations

  • Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, USA

    Michael J. May

Bibliographic Information

  • Book Title: NF-kappa B

  • Book Subtitle: Methods and Protocols

  • Editors: Michael J. May

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2422-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2421-9Published: 04 March 2015

  • Softcover ISBN: 978-1-4939-4811-6Published: 09 October 2016

  • eBook ISBN: 978-1-4939-2422-6Published: 03 March 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 668

  • Number of Illustrations: 56 b/w illustrations, 61 illustrations in colour

  • Topics: Biochemistry, general, Posttranslational Modification

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access