Skip to main content
  • Book
  • © 2015

Membrane Trafficking

Second Edition

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1270)

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (30 protocols)

  1. Front Matter

    Pages i-xv
  2. Biochemical and Molecular Genetics Approaches

    1. Front Matter

      Pages 13-13
    2. Import of Proteins into Isolated Yeast Mitochondria

      • Valentina Peleh, Ajay Ramesh, Johannes M. Herrmann
      Pages 37-50
    3. Fractionation of Plasmodium-Infected Human Red Blood Cells to Study Protein Trafficking

      • Simone Külzer, Verena Bittl, Jude M. Przyborski
      Pages 71-80
    4. Investigating Signaling Processes in Membrane Trafficking

      • Laura J. Sharpe, Andrew J. Brown
      Pages 81-90
    5. Recruitment of Coat Proteins to Liposomes and Peptidoliposomes

      • Sonja Huser, Gregor Suri, Pascal Crottet, Martin Spiess
      Pages 91-106
    6. A β-Lactamase Based Assay to Measure Surface Expression of Membrane Proteins

      • Pieter Beerepoot, Vincent M. Lam, Ali Salahpour
      Pages 107-114
    7. Cell-Free Reconstitution of Multivesicular Body (MVB) Cargo Sorting

      • Monica Gireud, Natalie Sirisaengtaksin, Susan Tsunoda, Andrew J. Bean
      Pages 115-124
    8. Analysis of Biogenesis of Lipid Droplets by Examining Rab40c Associating with Lipid Droplets

      • Ran Tan, Xiaohui Xu, Wanjin Hong, Tuanlao Wang
      Pages 125-135
    9. Assessing Mammalian Autophagy

      • Sharon A. Tooze, Hannah C. Dooley, Harold B. J. Jefferies, Justin Joachim, Delphine Judith, Christopher A. Lamb et al.
      Pages 155-165
    10. Expression of Functional Myc-Tagged Conserved Oligomeric Golgi (COG) Subcomplexes in Mammalian Cells

      • Rose A. Willett, Tetyana A. Kudlyk, Vladimir V. Lupashin
      Pages 167-177
    11. Understanding of Complex Protein Interactions with Respect to Anchorage Independence

      • Jill M. Tham, Sofie C. Van Huffel, Wanjin Hong
      Pages 205-225
    12. Application of Flow Cytometry to Analyze Intracellular Location and Trafficking of Cargo in Cell Populations

      • Wei Hong Toh, Fiona J. Houghton, Pei Zhi Cheryl Chia, Yasmin M. Ramdzan, Danny M. Hatters, Paul A. Gleeson
      Pages 227-238
    13. Approaches to Analyze the Role of Rab GTPases in Endocytic Trafficking of Epidermal Growth Factor Receptor (EGFR)

      • Christelle En Lin Chua, Angeline Yim Kiat Lim, Bor Luen Tang
      Pages 239-251

About this book

This second edition explores membrane trafficking research that has been at the frontier of cell and molecular biology. In the past 20 years, this field has witnessed a remarkable convergence and synergism from information gained using genetic approaches in yeast cells, and biochemical and molecular approaches in mammalian cells. This volume contains updated chapters and the inclusion of a large number of new chapters, and is divided into three parts. Part I includes biochemical and molecular genetics approaches and methods used in analysing membrane traffic, in both yeast and mammalian cell models. Part II focuses on imaging and microscopy approaches and techniques, while Part III highlights the Omics-type approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and resourceful, Membrane Trafficking, Second Edition, is a valuable resource for both experienced researchers, graduate students, and junior scientists in the field of membrane trafficking.

Editors and Affiliations

  • Department of Biochemistry, National University of Singapore, Singapore, Singapore

    Bor Luen Tang

Bibliographic Information

  • Book Title: Membrane Trafficking

  • Book Subtitle: Second Edition

  • Editors: Bor Luen Tang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2309-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2308-3Published: 22 February 2015

  • Softcover ISBN: 978-1-4939-4911-3Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2309-0Published: 21 February 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 457

  • Number of Illustrations: 36 b/w illustrations, 59 illustrations in colour

  • Topics: Cell Biology, Membrane Biology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access