Site-Specific Protein Labeling

This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Chapters provide protocols for labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols provides a comprehensive overview on the most relevant and established labeling methodologies, and helps researchers to choose the most appropriate labeling method for their biological question.

• CLIP-tag technology
• FlAsH-tag
• SNAP-tag
• intracellular proteins
• labeled proteins
• membrane translocation
• protein translation
• site-specific protein labeling
• tag-based methods
• unnatural amino acids
• Protein Structure

• Department of Chemistry, École Normale Supérieure, Paris, France

  Arnaud Gautier

• Sensitive Farbstoffe GbR, Munich, Germany

  Marlon J. Hinner

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