Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1262)
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Table of contents (21 protocols)
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Front Matter
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Imaging of Noncoding RNAs and Nuclear Bodies
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Front Matter
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Biochemical Analysis of Noncoding RNAs and Nuclear Bodies
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Front Matter
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Cell and Molecular Biological Approach Toward the Analysis of Noncoding RNAs and Nuclear Bodies
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Front Matter
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About this book
This volume focuses on cytological, biochemical, and molecular biological methods to identify and examine the function of each nuclear body, with an emphasis on the analysis of long non-coding RNAs. Chapters focus on exploring recent studies that reveal how certain long non protein-coding RNAs accumulate in specific nuclear bodies and regulate the function of the bodies by serving as architectural components or controlling the dynamics of associating protein components. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Nuclear Bodies and Noncoding RNAs: Methods and Protocols serves as a guideline for further study into the sophisticated regulation of gene expression.
Editors and Affiliations
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RNA Biology Laboratory, RIKEN, Saitama, Japan
Shinichi Nakagawa
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Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan
Tetsuro Hirose
Bibliographic Information
Book Title: Nuclear Bodies and Noncoding RNAs
Book Subtitle: Methods and Protocols
Editors: Shinichi Nakagawa, Tetsuro Hirose
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2253-6
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2252-9Published: 02 January 2015
Softcover ISBN: 978-1-4939-5549-7Published: 05 October 2016
eBook ISBN: 978-1-4939-2253-6Published: 02 January 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 351
Number of Illustrations: 24 b/w illustrations, 46 illustrations in colour
Topics: Human Genetics, Gene Function