Skip to main content
SpringerLink
Log in
Book cover

Insoluble Proteins

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Elena García-Fruitós

    1. Departament de Genètica i de Microbiologia, CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) and Universitat Autònoma de Barcelona, Bellaterra (Barcelona), Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols involving insoluble proteins
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1258)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (24 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins

    • Neus Ferrer-Miralles, Paolo Saccardo, José Luis Corchero, Zhikun Xu, Elena García-Fruitós
    Pages 1-24

  3. Recombinant Protein Production in Escherichia coli

    1. Front Matter

      Pages 25-25
      Download chapter PDF

    2. Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production

      • Agustín Correa, Pablo Oppezzo
      Pages 27-44

    3. Optimization of Culture Parameters and Novel Strategies to Improve Protein Solubility

      • Ranjana Arya, Jamal S. M. Sabir, Roop S. Bora, Kulvinder S. Saini
      Pages 45-63

    4. Cleavable Self-Aggregating Tags (cSAT) for Protein Expression and Purification

      • Zhanglin Lin, Qing Zhao, Bihong Zhou, Lei Xing, Wanghui Xu
      Pages 65-78

    5. Beyond the Cytoplasm of Escherichia coli: Localizing Recombinant Proteins Where You Want Them

      • Jason T. Boock, Dujduan Waraho-Zhmayev, Dario Mizrachi, Matthew P. DeLisa
      Pages 79-97

    6. Characterization of Amyloid-Like Properties in Bacterial Intracellular Aggregates

      • Anna Villar-Pique, Susanna Navarro, Salvador Ventura
      Pages 99-122

  4. Strategies to Produce Insoluble Proteins in Cell-Free Expression Systems

    1. Front Matter

      Pages 123-123
      Download chapter PDF

    2. Co-translational Stabilization of Insoluble Proteins in Cell-Free Expression Systems

      • Lei Kai, Erika Orbán, Erik Henrich, Davide Proverbio, Volker Dötsch, Frank Bernhard
      Pages 125-143

  5. Recombinant Protein Production in Lactic Acid Bacteria (LAB)

    1. Front Matter

      Pages 145-145
      Download chapter PDF

    2. Functional Expression of Plant Membrane Proteins in Lactococcus lactis

      • Sylvain Boutigny, Emeline Sautron, Annie Frelet-Barrand, Lucas Moyet, Daniel Salvi, Norbert Rolland et al.
      Pages 147-165

  6. Recombinant Protein Production in Yeast

    1. Front Matter

      Pages 167-167
      Download chapter PDF

    2. High Cell-Density Expression System: Yeast Cells in a Phalanx Efficiently Produce a Certain Range of “Difficult-to-Express” Secretory Recombinant Proteins

      • Yasuaki Kawarasaki, Takeshi Kurose, Keisuke Ito
      Pages 169-178

  7. Recombinant Protein Production in Insect Cells-Baculovirus

    1. Front Matter

      Pages 179-179
      Download chapter PDF

    2. Insect Cells–Baculovirus System for the Production of Difficult to Express Proteins

      • Judit Osz-Papai, Laura Radu, Wassim Abdulrahman, Isabelle Kolb-Cheynel, Nathalie Troffer-Charlier, Catherine Birck et al.
      Pages 181-205

  8. Recombinant Protein Production in Mammalian Cells

    1. Front Matter

      Pages 207-207
      Download chapter PDF

    2. Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins

      • Joanne E. Nettleship, Peter J. Watson, Nahid Rahman-Huq, Louise Fairall, Mareike G. Posner, Abhishek Upadhyay et al.
      Pages 209-222

    3. Recombinant Glycoprotein Production in Human Cell Lines

      • Kamilla Swiech, Marcela Cristina Corrêa de Freitas, Dimas Tadeu Covas, Virgínia Picanço-Castro
      Pages 223-240

  9. Recombinant Protein Production in Other Systems

    1. Front Matter

      Pages 241-241
      Download chapter PDF
Back to top

Keywords

About this book

With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Insoluble Proteins: Methods and Protocols aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate.

Editors and Affiliations

  • Departament de Genètica i de Microbiologia, CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) and Universitat Autònoma de Barcelona, Bellaterra (Barcelona), Spain

    Elena García-Fruitós

Bibliographic Information

  • Book Title: Insoluble Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Elena García-Fruitós

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2205-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2204-8Published: 02 December 2014

  • Softcover ISBN: 978-1-4939-4675-4Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2205-5Published: 01 December 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 425

  • Number of Illustrations: 31 b/w illustrations, 41 illustrations in colour

  • Topics: Protein Science

Publish with us

Policies and ethics

Back to top

Search

Navigation