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Dopamine Receptor Technologies

  • Book
  • © 2015

Overview

Editors:
  1. Mario Tiberi

    1. Ottawa Hospital Research Institute and University of Ottawa, Ottawa, Canada

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols involving dopamine receptor research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Neuromethods (NM, volume 96)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Genetic, Epigenetic, and Post-transcriptional Analysis of Dopamine Receptors

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Genetic and Epigenetic Methods for Analysis of the Dopamine D2 Receptor Gene

      • Laura M. Fiori, Mireille Daigle, Paul R. Albert
      Pages 3-12

    3. Characterization of D1 Dopamine Receptor Posttranscriptional Regulation

      • Eldo V. Kuzhikandathil
      Pages 13-27

  3. Computational and Biochemical Methods in the Investigation of Dopamine Receptor Structure, Binding, and Post-translational Regulation

    1. Front Matter

      Pages 29-29
      Download chapter PDF

    2. Computational Approaches in the Structure–Function Studies of Dopamine Receptors

      • Mayako Michino, Lei Shi
      Pages 31-42

    3. Cell-Free Protein Synthesis and Purification of the Dopamine D2 Receptor

      • Dipannita Basu, Ritesh Daya, Christal D. R. Sookram, Ram K. Mishra
      Pages 43-63

    4. Wnt Ligand Binding to and Regulation of Dopamine D2 Receptors

      • Sehyoun Yoon, Mi-Hyun Choi, Ja-Hyun Baik
      Pages 65-78

    5. Regulation of Pre- and Postsynaptic Protein Phosphorylation by Dopamine D2 Receptors

      • Alessandra Bonito-Oliva, Giada Spigolon, Gilberto Fisone
      Pages 79-100

    6. Study of Dopamine D1 Receptor Regulation by G Protein-Coupled Receptor Kinases Using Whole-Cell Phosphorylation and Cross-Linking Methods

      • Keyvan Sedaghat, Boyang Zhang, Xiaodi Yang, Caroline Lefebvre, Mario Tiberi
      Pages 101-138

    7. Ubiquitination of Dopamine Receptor Studied by Sequential Double Immunoprecipitation

      • Kamila Skieterska, Pieter Rondou, Kathleen Van Craenenbroeck
      Pages 139-156

  4. Visualization and Imaging of Dopamine Receptors and Ligands

    1. Front Matter

      Pages 157-157
      Download chapter PDF

    2. Dopamine Receptors in the Subthalamic Nucleus: Identification and Localization of D5 Receptors

      • Lionel Froux, Diana Suarez-Boomgaard, Jerome Baufreton, Alicia Rivera, Maurice Garret, Anne Taupignon
      Pages 159-176

    3. MALDI Mass Spectrometry Imaging of Dopamine and PET D1 and D2 Receptor Ligands in Rodent Brain Tissues

      • Richard J. A. Goodwin, Mohammadreza Shariatgorji, Per E. Andren
      Pages 177-196

    4. Positron Emission Tomography Imaging of Dopaminergic Receptors in Rats

      • Boguslaw Szczupak, Abraham Martín
      Pages 197-208

  5. Molecular and Cell Biological Approaches in the Study of Dopamine Receptor Function

    1. Front Matter

      Pages 209-209
      Download chapter PDF

    2. Transactivation of Receptor Tyrosine Kinases by Dopamine Receptors

      • Jeff S. Kruk, Azita Kouchmeshky, Nicholas Grimberg, Marina Rezkella, Michael A. Beazely
      Pages 211-227

    3. Dopamine Receptors in Human Embryonic Stem Cell Differentiation

      • Glenn S. Belinsky, Mandakini B. Singh, Katerina D. Oikonomou, Michele L. McGovern, Srdjan D. Antic
      Pages 229-249

    4. Calcium and Phospholipase Cβ Signaling Through Dopamine Receptors

      • Lani S. Chun, R. Benjamin Free, David R. Sibley
      Pages 251-263

    5. Intracellular Trafficking Assays for Dopamine D2-Like Receptors

      • Chengchun Min, Mei Zheng, Kyeong-Man Kim
      Pages 265-275
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Keywords

About this book

To foster a better understanding of dopamine receptor functionality, this detailed volume creates an interface between updated classical methods and new emerging technologies heretofore not available to new or seasoned researchers. Divided in five sections dedicated to experimental approaches investigating different facets of dopaminergic signal transduction, Dopamine Receptor Technologies covers epigenetic and post-transcriptional analysis, computational and biochemical techniques, visualization and imaging methods, molecular and cell biological tools, as well as behavioral assessment. The book, as a part of the popular Neuromethods series, provides insightful step-by-step protocols and methodological reviews that readers will find useful.

Practical and versatile, Dopamine Receptor Technologies seeks to aid researchers in developing new pharmacological tools to improve our knowledge of in vivo roles played by each receptor subtype and the synthesis of prospective lead compounds for drug discovery.

Editors and Affiliations

  • Ottawa Hospital Research Institute and University of Ottawa, Ottawa, Canada

    Mario Tiberi

Bibliographic Information

  • Book Title: Dopamine Receptor Technologies

  • Editors: Mario Tiberi

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-2196-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2195-9Published: 04 December 2014

  • Softcover ISBN: 978-1-4939-4889-5Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2196-6Published: 04 December 2014

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XIV, 379

  • Number of Illustrations: 37 b/w illustrations, 26 illustrations in colour

  • Topics: Neurosciences, Neurochemistry

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