Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1257)
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Table of contents (26 protocols)
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Front Matter
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Fundamental Aspects and Freezing Technology
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Front Matter
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Methods to Study Freezing and Drying Processes
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Front Matter
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Cryopreservation Protocols
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Front Matter
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About this book
This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.
Editors and Affiliations
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Institute of Multiphase Processes, Leibniz Universität Hannover, Hannover, Germany
Willem F. Wolkers
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Unit for Reproductive Medicine, Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany
Harriëtte Oldenhof
Bibliographic Information
Book Title: Cryopreservation and Freeze-Drying Protocols
Editors: Willem F. Wolkers, Harriëtte Oldenhof
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2193-5
Publisher: Springer New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2192-8Published: 27 November 2014
Softcover ISBN: 978-1-4939-4912-0Published: 22 September 2016
eBook ISBN: 978-1-4939-2193-5Published: 26 November 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XIII, 509
Number of Illustrations: 55 b/w illustrations, 35 illustrations in colour
Topics: Biotechnology, Biomaterials