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  • © 2015

Neuronal Cell Death

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1254)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
  2. Neuronal Cell Death: An Overview of Its Different Forms in Central and Peripheral Neurons

    • Laura Lossi, Claudia Castagna, Adalberto Merighi
    Pages 1-18
  3. Molecular, Structural, Functional, and Genomic Changes in Dying Neurons

    1. Front Matter

      Pages 19-19
    2. Nuclear Signs of Pre-neurodegeneration

      • Fernando C. Baltanás, Jorge Valero, Jose Ramón Alonso, Maria Teresa Berciano, Miguel Lafarga
      Pages 43-54
    3. Calcium Imaging in Neuron Cell Death

      • María Calvo, Carlos Villalobos, Lucía Núñez
      Pages 73-85
    4. Real-Time Visualization of Caspase-3 Activation by Fluorescence Resonance Energy Transfer (FRET)

      • Silvia Alasia, Carolina Cocito, Adalberto Merighi, Laura Lossi
      Pages 99-113
    5. High-Throughput Cell Death Assays

      • Matthew E. Pamenter, Gabriel G. Haddad
      Pages 153-163
  4. Cell Death in Neuropathology and Experimental Neuropathology

    1. Front Matter

      Pages 165-165
    2. Image Analysis Algorithms for Immunohistochemical Assessment of Cell Death

      • Stan Krajewski, Jeffrey Wang, Tashmia Khan, Jonathan Liu, Chia-Hung Sze, Maryla Krajewska
      Pages 181-196
    3. In Vitro Oxygen-Glucose Deprivation to Study Ischemic Cell Death

      • Carla I. Tasca, Tharine Dal-Cim, Helena Cimarosti
      Pages 197-210
    4. Laser Microbeam Targeting of Single Nerve Axons in Cell Culture

      • Nicholas Hyun, Linda Z. Shi, Michael W. Berns
      Pages 211-226
    5. Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser Ophthalmoscopy

      • Eduardo M. Normando, Mohammad H. Dehabadi, Li Guo, Lisa A. Turner, Gaia Pollorsi, M. Francesca Cordeiro
      Pages 227-237

About this book

This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of primary interest in neuropathology and in experimental neuropathology, a series of gene engineering techniques to obtain and manipulate neuronal stem cells and progenitors, to prepare HSV-1 vectors for the gene therapy, and to CNS transplantation of bone marrow stem cells, and finally, some very interesting protocols for the study of cell death in non-mammalian models. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Neuronal Cell Death: Methods and Protocols seeks to serve a large audience of scientists that are currently active in the field or are willing to enter such an exciting and still expanding area of neurobiology.

Editors and Affiliations

  • Dept. of Veterinary Sciences, University of Torino, Grugliasco, Italy

    Laura Lossi, Adalberto Merighi

Bibliographic Information

  • Book Title: Neuronal Cell Death

  • Book Subtitle: Methods and Protocols

  • Editors: Laura Lossi, Adalberto Merighi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2152-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2151-5Published: 28 November 2014

  • Softcover ISBN: 978-1-4939-4464-4Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2152-2Published: 27 November 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 396

  • Number of Illustrations: 34 b/w illustrations, 48 illustrations in colour

  • Topics: Neurosciences, Apoptosis

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access