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  • © 2015

Extracellular Matrix

  • Includes cutting-edge methods and protocols involving the extracellular matrix in native, injured, and engineered neural tissues
  • Provides step-by-step detail for the utmost clarity
  • Contains some key implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 93)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
  2. In Vitro Models

    1. Front Matter

      Pages 33-33
    2. Isolation of Perinatal Mouse Cerebral Cortical Astrocytes

      • Rolicia F. Martin, Elizabeth M. Powell
      Pages 35-43
    3. Extracellular Matrix Components as a Substrate for Outgrowing Motoneurons

      • Rebecca Conrad, Alice Klausmeyer, Teresa Tsai, Andreas Faissner, Stefan Wiese
      Pages 67-78
    4. Culturing Neurons, Glia, and Progenitor Cells in Three-Dimensional Hydrogels

      • Swarnalatha Balasubramanian, Elizabeth M. Powell, Jennie B. Leach
      Pages 91-99
    5. Investigating Cell-ECM Interactions and ECM Synthesis in Three-Dimensional Hydrogels

      • Swarnalatha Balasubramanian, Elizabeth M. Powell, Jennie B. Leach
      Pages 101-109
  3. Injury, Degeneration, and Regeneration

    1. Front Matter

      Pages 111-111
    2. Chondroitinase Gene Therapy for Spinal Cord Injury

      • Jianli Hu, Gabrielle M. Curinga, George M. Smith
      Pages 139-149
  4. Therapeutics and Engineered Systems

    1. Front Matter

      Pages 161-161
    2. Evaluating the Spatial and Temporal Protein Production in Neural Tissue Engineering Constructs In Vitro

      • Caroline P. Addington, Christine Pauken, Sarah E. Stabenfeldt
      Pages 163-179
    3. Cultivation and Imaging of Astrocytes on Protein-Coated Fluorescent Topographies Constructed from Aligned PLLA Electrospun Fibers

      • Jonathan M. Zuidema, María C. Hyzinski-García, Alexander A. Mongin, Ryan J. Gilbert
      Pages 181-195

About this book

This volume provides comprehensive procedures for analyzing the extracellular matrix in native, injured, and engineered neural tissues. Divided into four parts, each focusing on different aspects of the extracellular matrix and the nervous system, Extracellular Matrix covers methods to analyze native tissue, in vitro models for investigating cell-extracellular matrix interactions in a variety of contexts, protocols to investigate the role of the extracellular matrix in nervous system injury, degeneration, and regeneration, as well as therapeutics and engineered systems. Each chapter is written by leading experts and presents established protocols in a concise format, encompassing current technologies as well as methods developed over years of research. Beginning with an introduction to the method, chapters continue with a listing of the materials and equipment, step-by-step protocols, and a Notes section with troubleshooting tips, supplemental details and alternative approaches, as well as a list of references for further information.

As part of the practical and convenient Neuromethods series, Extracellular Matrix serves as an invaluable aid for researchers studying this vital area of neuroscience.

Editors and Affiliations

  • University of Maryland, Baltimore County, Baltimore, USA

    Jennie B. Leach

  • Department of Anatomy & Neurobiology, University of Maryland School of Medicine, Baltimore, USA

    Elizabeth M. Powell

Bibliographic Information

  • Book Title: Extracellular Matrix

  • Editors: Jennie B. Leach, Elizabeth M. Powell

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-2083-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2082-2Published: 04 November 2014

  • Softcover ISBN: 978-1-4939-4687-7Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2083-9Published: 03 November 2014

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XII, 229

  • Number of Illustrations: 24 b/w illustrations, 23 illustrations in colour

  • Topics: Neurosciences, Neurobiology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access