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  • © 2015

Advanced Fluorescence Microscopy

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1251)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
  2. The Physical Basis of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular Applications

    • Natalie S. Poulter, William T. E. Pitkeathly, Philip J. Smith, Joshua Z. Rappoport
    Pages 1-23
  3. Two-Photon Excitation Microscopy and Its Applications in Neuroscience

    • Ricardo Mostany, Amaya Miquelajauregui, Matthew Shtrahman, Carlos Portera-Cailliau
    Pages 25-42
  4. Live Spheroid Formation Recorded with Light Sheet-Based Fluorescence Microscopy

    • Francesco Pampaloni, Roli Richa, Nariman Ansari, Ernst H. K. Stelzer
    Pages 43-57
  5. Fluorescence Microscopy-Based RNA Interference Screening

    • Manuel Gunkel, Nina Beil, Jürgen Beneke, Jürgen Reymann, Holger Erfle
    Pages 59-66
  6. Fluorescence Resonance Energy Transfer Microscopy (FRET)

    • Katarzyna M. Kedziora, Kees Jalink
    Pages 67-82
  7. Analysis of Biomolecular Dynamics by FRAP and Computer Simulation

    • Bart Geverts, Martin E. van Royen, Adriaan B. Houtsmuller
    Pages 109-133
  8. Fluorescence Correlation Spectroscopy

    • Mark A. Hink
    Pages 135-150
  9. Homo-FRET Imaging Highlights the Nanoscale Organization of Cell Surface Molecules

    • Suvrajit Saha, Riya Raghupathy, Satyajit Mayor
    Pages 151-173
  10. Practical Structured Illumination Microscopy

    • E. Hesper Rego, Lin Shao
    Pages 175-192
  11. 4Pi Microscopy of the Nuclear Pore Complex

    • Martin Kahms, Jana Hüve, Reiner Peters
    Pages 193-211
  12. Application of STED Microscopy to Cell Biology Questions

    • Natalia H. Revelo, Silvio O. Rizzoli
    Pages 213-230
  13. Three-Dimensional Photoactivated Localization Microscopy with Genetically Expressed Probes

    • Kelsey Temprine, Andrew G. York, Hari Shroff
    Pages 231-261
  14. Direct Stochastic Optical Reconstruction Microscopy (dSTORM)

    • Ulrike Endesfelder, Mike Heilemann
    Pages 263-276
  15. Back Matter

    Pages 291-294

About this book

This volume provides an overview of advanced  fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems.  Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge,  Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.

Editors and Affiliations

  • Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany

    Peter J. Verveer

Bibliographic Information

  • Book Title: Advanced Fluorescence Microscopy

  • Book Subtitle: Methods and Protocols

  • Editors: Peter J. Verveer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2080-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2079-2Published: 13 November 2014

  • Softcover ISBN: 978-1-4939-4252-7Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2080-8Published: 12 November 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 294

  • Number of Illustrations: 21 b/w illustrations, 65 illustrations in colour

  • Topics: Imaging / Radiology, Biological Microscopy

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access