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Plant Cell Expansion

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. José M. Estevez

    1. Departamento de Fisiología Biologia Molecular y Celular (DFBMC), University of Buenos Aires IFIByNE-CONICET, Buenos Aires, Argentina

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1242)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Penium margaritaceum as a Model Organism for Cell Wall Analysis of Expanding Plant Cells

    • Maja G. Rydahl, Jonatan U. Fangel, Maria Dalgaard Mikkelsen, I. Elisabeth Johansen, Amanda Andreas, Jesper Harholt et al.
    Pages 1-21

  3. Using Chemical Genomics to Study Cell Wall Formation and Cell Growth in Arabidopsis thaliana and Penium margaritaceum

    • N. Worden, V. Esteva Esteve, D. S. Domozych, G. Drakakaki
    Pages 23-39

  4. Optimized Method for Growing In Vitro Arabidopsis thaliana Pollen Tubes

    • Cecilia Borassi, Juliana Pérez Di Giorgio, María R. Scarpin, Jorge Muschietti, José M. Estevez
    Pages 41-47

  5. Imaging of Calcium Dynamics in Pollen Tube Cytoplasm

    • María Laura Barberini, Jorge Muschietti
    Pages 49-57

  6. Live Imaging of Root Hairs

    • Silvia M. Velasquez, Jose R. Dinneny, José M. Estevez
    Pages 59-66

  7. Improved ROS Measurement in Root Hair Cells

    • Silvina Paola Denita Juárez, Silvina Mangano, José M. Estevez
    Pages 67-71

  8. A Root Hair Assay to Expedite Cell Death Research

    • Joanna Kacprzyk, Paul F. McCabe
    Pages 73-82

  9. Vacuolar Staining Methods in Plant Cells

    • David Scheuring, Maria Schöller, Jürgen Kleine-Vehn, Christian Löfke
    Pages 83-92

  10. Live Cell Imaging of FM4-64, a Tool for Tracing the Endocytic Pathways in Arabidopsis Root Cells

    • Adeline Rigal, Siamsa M. Doyle, Stéphanie Robert
    Pages 93-103

  11. Salt-Stress Regulation of Root System Growth and Architecture in Arabidopsis Seedlings

    • Lina Duan, Jose Sebastian, Jose R. Dinneny
    Pages 105-122

  12. Quantification of Fluorescent Reporters in Plant Cells

    • Michael Pound, Andrew P. French, Darren M. Wells
    Pages 123-131

  13. Live Cell Imaging of the Cytoskeleton and Cell Wall Enzymes in Plant Cells

    • Arun Sampathkumar, Raymond Wightman
    Pages 133-141

  14. Using the Split-Ubiquitin Yeast Two-Hybrid System to Test Protein–Protein Interactions of Transmembrane Proteins

    • Logan Bashline, Ying Gu
    Pages 143-158

  15. Activation Tag Screening for Cell Expansion Genes in Arabidopsis thaliana

    • Chaowen Xiao, Charles T. Anderson
    Pages 159-171

  16. BiFC for Protein–Protein Interactions and Protein Topology: Discussing an Integrative Approach for an Old Technique

    • Giovanni Stefano, Luciana Renna, Federica Brandizzi
    Pages 173-182

  17. N-Glycosylation and Plant Cell Growth

    • Christiane Veit, Ulrike Vavra, Richard Strasser
    Pages 183-194

  18. Peptide Separation Methodologies for In-depth Proteomics

    • Sajad Majeed Zargar, Rie Kurata, Randeep Rakwal, Yoichiro Fukao
    Pages 195-209

  19. Structural and Mechanical Characterization of Growing Arabidopsis Plant Cell Walls

    • Friederike Saxe, Ingo Burgert, Michaela Eder
    Pages 211-227

  20. Back Matter

    Pages 229-231
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Keywords

About this book

This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Chapters focus on the cutting-edge methods to study in detail several complex aspects of cell expansion such as secretion, endocytosis and recycling, cellular signaling and trafficking, and protein and polysaccharides cell wall biosynthesis in real time during cell expansion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Cell Expansion: Methods and Protocols is an essential reference book for plant scientist, molecular, and cell biologist as well as plant biochemists​.

Editors and Affiliations

  • Departamento de Fisiología Biologia Molecular y Celular (DFBMC), University of Buenos Aires IFIByNE-CONICET, Buenos Aires, Argentina

    José M. Estevez

Bibliographic Information

  • Book Title: Plant Cell Expansion

  • Book Subtitle: Methods and Protocols

  • Editors: José M. Estevez

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1902-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1901-7Published: 19 November 2014

  • Softcover ISBN: 978-1-4939-5381-3Published: 22 September 2016

  • eBook ISBN: 978-1-4939-1902-4Published: 18 November 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 231

  • Number of Illustrations: 8 b/w illustrations, 41 illustrations in colour

  • Topics: Plant Sciences, Plant Physiology

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